HitPredict

Interactor 1: SUMO1 (P63165)
Small ubiquitin-related modifier 1 (SUMO-1) (GAP-modifying protein 1) (GMP1) (SMT3 homolog 3) (Sentrin) (Ubiquitin-homology domain protein PIC1) (Ubiquitin-like protein SMT3C) (Smt3C) (Ubiquitin-like protein UBL1)
Gene: SUMO1

Interactor 2: PIAS3 (Q9Y6X2)
E3 SUMO-protein ligase PIAS3 (EC 2.3.2.-) (E3 SUMO-protein transferase PIAS3) (Protein inhibitor of activated STAT protein 3)
Gene: PIAS3

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.78	1.00	0.888

Property	Details
Experiments	15569683, 35044719, 19217413, 16524884
Identification Methods	two hybrid, unspecified method, pull down, phage display
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	Rad60-SLD~zf-MIZ
Common GO terms	GO:0005654 - nucleoplasm GO:0016607 - nuclear speck GO:0016925 - protein sumoylation
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 101 Hs			1 628
+	Hs
		902827 SUMO2_HUMAN (1-97): 1.77e-38: 125: 45.36		PIAS3_HUMAN (1-628): 0: 879: 100.00
+	Hs
		879167 SUMO3_HUMAN (1-97): 1.65e-37: 123: 47.42		PIAS3_HUMAN (1-628): 0: 879: 100.00
+	Mm
		222291 SUMO1_MOUSE (1-101): 1.01e-41: 134: 100.00		PIAS3_MOUSE (1-628): 0: 867: 96.98
+	Hs
		278040 SUMO1_HUMAN (1-101): 1.01e-41: 134: 100.00		PIAS1_HUMAN (1-628): 0: 728: 56.69
+	Hs
		102146 SUMO5_HUMAN (1-101): 4.52e-41: 132: 88.12		PIAS1_HUMAN (1-628): 0: 728: 56.69
+	Hs
		278020 SUMO2_HUMAN (1-97): 1.77e-38: 125: 45.36		PIAS1_HUMAN (1-628): 0: 728: 56.69
+	Hs
		278009 SUMO3_HUMAN (1-97): 1.65e-37: 123: 47.42		PIAS1_HUMAN (1-628): 0: 728: 56.69
+	Hs
		278256 SUMO1_HUMAN (1-101): 1.01e-41: 134: 100.00		PIAS2_HUMAN (1-550): 0: 651: 59.00
+	Hs
		102147 SUMO5_HUMAN (1-101): 4.52e-41: 132: 88.12		PIAS2_HUMAN (1-550): 0: 651: 59.00
+	Hs
		278253 SUMO2_HUMAN (1-97): 1.77e-38: 125: 45.36		PIAS2_HUMAN (1-550): 0: 651: 59.00
+	Hs
		278250 SUMO3_HUMAN (1-97): 1.65e-37: 123: 47.42		PIAS2_HUMAN (1-550): 0: 651: 59.00
+	Rn
		1140374 SUMO3_RAT (1-100): 1.5e-39: 128: 46.00		PIAS2_RAT (1-528): 0: 626: 59.71
+	Hs
		928135 SUMO1_HUMAN (1-101): 1.01e-41: 134: 100.00		PIAS4_HUMAN (2-477): 2.11e-177: 514: 46.71
+	Rn
		78199 SUMO1_RAT (1-101): 1.01e-41: 134: 100.00		B5DFF9_RAT (2-443): 7.68e-174: 505: 50.45
+	Hs
		902668 SUMO2_HUMAN (1-97): 1.77e-38: 125: 45.36		PIAS4_HUMAN (2-477): 2.11e-177: 514: 46.71
+	Hs
		879136 SUMO3_HUMAN (1-97): 1.65e-37: 123: 47.42		PIAS4_HUMAN (2-477): 2.11e-177: 514: 46.71
+	Rn
		78200 SUMO3_RAT (1-100): 1.5e-39: 128: 46.00		B5DFF9_RAT (2-443): 7.68e-174: 505: 50.45
+	At
		1147334 SUMO2_ARATH (6-99): 5.11e-38: 124: 45.75		SIZ1_ARATH (5-623): 1.75e-120: 380: 21.29
+	At
		1147333 SUMO3_ARATH (6-97): 1.88e-37: 123: 38.04		SIZ1_ARATH (5-623): 1.75e-120: 380: 21.29
+	Rn
		89025 SUMO3_RAT (1-100): 1.5e-39: 128: 46.00		D4AE97_RAT (79-422): 2.72e-84: 287: 24.93