HitPredict

Interactor 1: UB2L3 (P68036)
Ubiquitin-conjugating enzyme E2 L3 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme L3) (L-UBC) (UbcH7) (Ubiquitin carrier protein L3) (Ubiquitin-conjugating enzyme E2-F1) (Ubiquitin-protein ligase L3)
Gene: UBE2L3

Interactor 2: ITCH (Q96J02)
E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
Gene: ITCH

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.97	1.00	0.988

Property	Details
Experiments	26245901, 29643511, 27182664, 17996703, 25632008, 25723849, 26949039, 29212706, 29515120, 29378950, 30017913, 17719543, 34999730, 22009753, 18718449, 15350225, 17592138, 17038327, 15678106, 20068034
Identification Methods	enzymatic study, pull down, affinity chromatography technology
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	HECT~UQ_con
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0006511 - ubiquitin-dependent protein catabolic process and 1 others more... GO:0016567 - protein ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 154 Hs			1 903
+	Hs
		1294888 UB2D4_HUMAN (2-149): 6.75e-76: 224: 38.93		ITCH_HUMAN (1-903): 0: 926: 100.00
+	Hs
		849411 UB2D1_HUMAN (2-149): 5.66e-75: 221: 38.26		ITCH_HUMAN (1-903): 0: 926: 100.00
+	Hs
		919827 UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58		ITCH_HUMAN (1-903): 0: 926: 100.00
+	Hs
		919860 UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		125808 UB2D4_HUMAN (2-149): 6.75e-76: 224: 38.93		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		125262 UB2D1_HUMAN (2-149): 5.66e-75: 221: 38.26		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		125335 UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		849416 UB2D1_HUMAN (2-149): 5.66e-75: 221: 38.26		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Hs
		919834 UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Hs
		808052 UB2D1_HUMAN (2-149): 5.66e-75: 221: 38.26		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		808078 UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		849353 UB2D1_HUMAN (2-149): 5.66e-75: 221: 38.26		HUWE1_HUMAN (415-902): 1.81e-160: 523: 34.88
+	Hs
		919765 UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58		HUWE1_HUMAN (415-902): 1.81e-160: 523: 34.88
+	Hs
		919782 UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58		HACE1_HUMAN (376-897): 4.35e-154: 478: 30.61
+	Hs
		919869 UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		919867 UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Sc
		549815 UBC4_YEAST (1-148): 8.58e-75: 221: 40.27		RSP5_YEAST (16-440): 9.75e-40: 160: 19.07
+	Hs
		919889 UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58		HECW2_HUMAN (1-512): 1.38e-13: 77.2: 11.34
+	Hs
		849351 UB2D1_HUMAN (2-149): 5.66e-75: 221: 38.26		HECW1_HUMAN (26-479): 2.25e-13: 76.9: 10.76
+	Hs
		919762 UB2D2_HUMAN (2-149): 7.95e-75: 221: 37.58		HECW1_HUMAN (26-479): 2.25e-13: 76.9: 10.76