HitPredict

Interactor 1: SRPK2 (P78362)
SRSF protein kinase 2 (EC 2.7.11.1) (SFRS protein kinase 2) (Serine/arginine-rich protein-specific kinase 2) (SR-protein-specific kinase 2) [Cleaved into: SRSF protein kinase 2 N-terminal; SRSF protein kinase 2 C-terminal]
Gene: SRPK2

Interactor 2: SRSF8 (Q9BRL6)
Serine/arginine-rich splicing factor 8 (Pre-mRNA-splicing factor SRP46) (Splicing factor SRp46) (Splicing factor, arginine/serine-rich 2B)
Gene: SRSF8

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.88	1.00	0.941

Property	Details
Experiments	34133714, 32296183, 33961781, 23602568, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, enzymatic study, two hybrid array, two hybrid prey pooling approach, validated two hybrid, anti tag coimmunoprecipitation, tandem affinity purification, protein kinase assay
Interaction Type	direct interaction, physical association, association, phosphorylation reaction

Property	Details
Interacting Pfam domains	Pkinase~RRM_1
Common GO terms	GO:0003723 - RNA binding GO:0005654 - nucleoplasm GO:0005829 - cytosol GO:0016607 - nuclear speck
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 688 Hs			1 282
+	Hs
		942600 SRPK2_HUMAN (12-688): 3.2e-128: 397: 95.66		SRSF2_HUMAN (1-100): 1.3e-41: 144: 80.00
+	Hs
		1302010 SRPK1_HUMAN (27-688): 1.57e-125: 388: 53.22		SRSF8_HUMAN (1-282): 5.34e-41: 144: 100.00
+	Hs
		964049 SRPK1_HUMAN (27-688): 1.57e-125: 388: 53.22		SRSF2_HUMAN (1-100): 1.3e-41: 144: 80.00
+	Hs
		942631 SRPK2_HUMAN (12-688): 3.2e-128: 397: 95.66		PABP4_HUMAN (2-103): 9.14e-22: 96.7: 19.61
+	Hs
		516460 SRPK2_HUMAN (12-688): 3.2e-128: 397: 95.66		PABP1_HUMAN (2-103): 1.99e-20: 92.9: 18.63
+	Hs
		516651 SRPK1_HUMAN (27-688): 1.57e-125: 388: 53.22		PABP1_HUMAN (2-103): 1.99e-20: 92.9: 18.63
+	Sc
		694216 SKY1_YEAST (1-686): 9.62e-118: 371: 30.50		PUB1_YEAST (12-99): 1.78e-17: 83.7: 20.46
+	Hs
		625400 CDK2_HUMAN (378-687): 1.93e-91: 288: 17.14		ELAV2_HUMAN (2-99): 7.35e-24: 100: 22.45
+	Hs
		516362 CDK2_HUMAN (378-687): 1.93e-91: 288: 17.14		PABP1_HUMAN (2-103): 1.99e-20: 92.9: 18.63
+	Hs
		964081 SRPK3_HUMAN (450-687): 1.19e-63: 223: 66.39		SRSF2_HUMAN (1-100): 1.3e-41: 144: 80.00
+	Hs
		833957 CLK2_HUMAN (62-229): 1.41e-63: 221: 33.73		SRSF8_HUMAN (1-282): 5.34e-41: 144: 100.00
+	Hs
		834109 CLK3_HUMAN (62-240): 6.66e-62: 220: 31.11		SRSF8_HUMAN (1-282): 5.34e-41: 144: 100.00
+	Hs
		833805 CLK1_HUMAN (52-226): 1.37e-61: 215: 31.25		SRSF8_HUMAN (1-282): 5.34e-41: 144: 100.00
+	Hs
		833874 CLK2_HUMAN (62-229): 1.41e-63: 221: 33.73		ELAV2_HUMAN (2-99): 7.35e-24: 100: 22.45
+	Hs
		834030 CLK3_HUMAN (62-240): 6.66e-62: 220: 31.11		ELAV2_HUMAN (2-99): 7.35e-24: 100: 22.45
+	Hs
		834033 CLK3_HUMAN (62-240): 6.66e-62: 220: 31.11		PABP4_HUMAN (2-103): 9.14e-22: 96.7: 19.61
+	Hs
		516416 CLK2_HUMAN (62-229): 1.41e-63: 221: 33.73		PABP1_HUMAN (2-103): 1.99e-20: 92.9: 18.63
+	Hs
		516417 CLK3_HUMAN (62-240): 6.66e-62: 220: 31.11		PABP1_HUMAN (2-103): 1.99e-20: 92.9: 18.63
+	Dm
		834186 DOA_DROME (519-687): 7.73e-14: 77: 29.12		Q9V3T8_DROME (5-181): 7.07e-31: 115: 50.83