HitPredict

Interactor 1: EWS (Q01844)
RNA-binding protein EWS (EWS oncogene) (Ewing sarcoma breakpoint region 1 protein)
Gene: EWSR1

Interactor 2: SMAD4 (Q13485)
Mothers against decapentaplegic homolog 4 (MAD homolog 4) (Mothers against DPP homolog 4) (Deletion target in pancreatic carcinoma 4) (SMAD family member 4) (SMAD 4) (Smad4) (hSMAD4)
Gene: SMAD4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.70	0.60	0.650

Property	Details
Experiments	16189514, 31515488, 29892012
Identification Methods	two hybrid, two hybrid array, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0006355 - regulation of DNA-templated transcription GO:0046872 - metal ion binding
Homologous interactions	9

Homologous interactions

Color coding for hit scores

	1 656 Hs			1 552
+	Hs
		358510 CO1A1_HUMAN (2-506): 1.09e-18: 92.9: 14.74		SMAD4_HUMAN (1-552): 0: 696: 100.00
+	Mm
		503173 CO1A1_MOUSE (4-312): 1.37e-09: 63.7: 8.92		SMAD4_MOUSE (1-552): 0: 688: 98.37
+	Hs
		967203 EWS_HUMAN (1-656): 1.54e-47: 179: 100.00		SMAD1_HUMAN (20-546): 0: 542: 34.91
+	Hs
		358494 CO1A1_HUMAN (2-506): 1.09e-18: 92.9: 14.74		SMAD3_HUMAN (19-549): 1.96e-176: 506: 31.22
+	Hs
		358519 CO1A1_HUMAN (2-506): 1.09e-18: 92.9: 14.74		SMAD2_HUMAN (19-549): 1.81e-166: 482: 28.65
+	Mm
		503178 CO1A1_MOUSE (4-312): 1.37e-09: 63.7: 8.92		SMAD3_MOUSE (19-549): 1.96e-176: 506: 31.22
+	Mm
		503177 CO1A1_MOUSE (4-312): 1.37e-09: 63.7: 8.92		SMAD2_MOUSE (19-549): 1.86e-167: 485: 28.65
+	Hs
		967541 EWS_HUMAN (1-656): 1.54e-47: 179: 100.00		PCLO_HUMAN (133-321): 1.93e-09: 63: 14.73
+	Mm
		192481 CO1A1_MOUSE (4-312): 1.37e-09: 63.7: 8.92		SMAD7_MOUSE (33-137): 2.38e-25: 110: 28.45