HitPredict

Interactor 1: TF65 (Q04206)
Transcription factor p65 (Nuclear factor NF-kappa-B p65 subunit) (Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
Gene: RELA

Interactor 2: IKBB (Q15653)
NF-kappa-B inhibitor beta (NF-kappa-BIB) (I-kappa-B-beta) (IkB-B) (IkB-beta) (IkappaBbeta) (Thyroid receptor-interacting protein 9) (TR-interacting protein 9) (TRIP-9)
Gene: NFKBIB

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.97	1.00	0.988

Property	Details
Experiments	27609421, 10498867, 8816457, 35271311, 22081069, 8887627, 26186194, 28514442, 12672800, 33961781, 21988832, 14743216, 14685242, 9135156, 16792530, 16226509
Identification Methods	affinity chromatography technology, two hybrid, pull down, tandem affinity purification, anti tag coimmunoprecipitation, electrophoretic mobility supershift assay, anti bait coimmunoprecipitation, two hybrid array
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Ank~RHD_dimer, Ank_2~RHD_dimer
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0006351 - DNA-templated transcription GO:0006954 - inflammatory response GO:0071222 - cellular response to lipopolysaccharide
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 551 Hs			1 356
+	Hs
		639733 TF65_HUMAN (1-551): 0: 601: 100.00		IKBA_HUMAN (14-335): 5.39e-78: 243: 30.21
+	Hs
		961368 TF65_HUMAN (1-551): 0: 601: 100.00		NFKB2_HUMAN (35-347): 1.91e-71: 240: 31.15
+	Mm
		978962 TF65_MOUSE (1-551): 0: 577: 87.88		IKBB_MOUSE (1-355): 1.52e-80: 251: 83.52
+	Rn
		1144369 Q7TQN4_RAT (1-551): 0: 588: 90.05		IKBA_RAT (7-335): 3.8e-76: 238: 30.77
+	Mm
		978991 TF65_MOUSE (1-551): 0: 577: 87.88		IKBA_MOUSE (7-335): 8.92e-77: 240: 31.07
+	Mm
		978989 TF65_MOUSE (1-551): 0: 577: 87.88		NFKB2_MOUSE (55-347): 8.38e-68: 230: 33.11
+	Hs
		121585 TF65_HUMAN (1-551): 0: 601: 100.00		IKBE_HUMAN (25-333): 1.22e-60: 204: 31.72
+	Mm
		222601 TF65_MOUSE (1-551): 0: 577: 87.88		IKBE_MOUSE (51-333): 1.19e-64: 211: 32.86
+	Hs
		981586 REL_HUMAN (12-523): 3.56e-165: 484: 41.64		IKBB_HUMAN (1-356): 9.91e-80: 249: 100.00
+	Hs
		639734 REL_HUMAN (12-523): 3.56e-165: 484: 41.64		IKBA_HUMAN (14-335): 5.39e-78: 243: 30.21
+	Hs
		961369 REL_HUMAN (12-523): 3.56e-165: 484: 41.64		NFKB2_HUMAN (35-347): 1.91e-71: 240: 31.15
+	Mm
		638475 NFKB1_MOUSE (7-451): 3.1e-157: 475: 30.19		IKBA_MOUSE (7-335): 8.92e-77: 240: 31.07
+	Mm
		638473 NFKB1_MOUSE (7-451): 3.1e-157: 475: 30.19		NFKB2_MOUSE (55-347): 8.38e-68: 230: 33.11
+	Hs
		121586 REL_HUMAN (12-523): 3.56e-165: 484: 41.64		IKBE_HUMAN (25-333): 1.22e-60: 204: 31.72
+	Hs
		587542 TF65_HUMAN (1-551): 0: 601: 100.00		NFKB1_HUMAN (28-140): 2.07e-10: 64.4: 29.57
+	Mm
		638449 TF65_MOUSE (1-551): 0: 577: 87.88		NFKB1_MOUSE (14-140): 5.94e-11: 65.9: 29.46
+	Xl
		981811 TF65_XENLA (13-549): 7.57e-176: 508: 51.53		Q6DCW3_XENLA (48-177): 4.22e-12: 68.2: 22.31
+	Hs
		587543 REL_HUMAN (12-523): 3.56e-165: 484: 41.64		NFKB1_HUMAN (28-140): 2.07e-10: 64.4: 29.57
+	Mm
		638435 NFKB1_MOUSE (7-451): 3.1e-157: 475: 30.19		NFKB1_MOUSE (14-140): 5.94e-11: 65.9: 29.46
+	Mm
		546805 REL_MOUSE (12-512): 1.43e-160: 471: 41.22		NFKB1_MOUSE (14-140): 5.94e-11: 65.9: 29.46