HitPredict

Interactor 1: TRI25 (Q14258)
E3 ubiquitin/ISG15 ligase TRIM25 (EC 6.3.2.n3) (Estrogen-responsive finger protein) (RING finger protein 147) (RING-type E3 ubiquitin transferase) (EC 2.3.2.27) (RING-type E3 ubiquitin transferase TRIM25) (Tripartite motif-containing protein 25) (Ubiquitin/ISG15-conjugating enzyme TRIM25) (Zinc finger protein 147)
Gene: TRIM25

Interactor 2: UB2D4 (Q9Y2X8)
Ubiquitin-conjugating enzyme E2 D4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme D4) (HBUCE1) (Ubiquitin carrier protein D4) (Ubiquitin-protein ligase D4)
Gene: UBE2D4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.48	1.00	0.698

Property	Details
Experiments	19549727
Identification Methods	two hybrid, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_UBOX
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0006511 - ubiquitin-dependent protein catabolic process GO:0070936 - protein K48-linked ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 630 Hs			1 147
+	Hs
		849330 TRI25_HUMAN (1-630): 3.52e-149: 447: 100.00		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		919727 TRI25_HUMAN (1-630): 3.52e-149: 447: 100.00		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		895794 TRI25_HUMAN (1-630): 3.52e-149: 447: 100.00		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		896442 TRI25_HUMAN (1-630): 3.52e-149: 447: 100.00		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Hs
		175730 TRI18_HUMAN (4-629): 4.7e-124: 383: 22.17		UB2D4_HUMAN (1-147): 8.89e-84: 243: 100.00
+	Hs
		175653 TRI18_HUMAN (4-629): 4.7e-124: 383: 22.17		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		175664 TRI18_HUMAN (4-629): 4.7e-124: 383: 22.17		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		175658 TRI18_HUMAN (4-629): 4.7e-124: 383: 22.17		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		1151762 Q6GX22_HUMAN (4-629): 2.93e-118: 368: 21.72		UB2D4_HUMAN (1-147): 8.89e-84: 243: 100.00
+	Hs
		175710 TRI18_HUMAN (4-629): 4.7e-124: 383: 22.17		UB2E2_HUMAN (4-146): 3.5e-75: 224: 64.34
+	Hs
		175703 TRI18_HUMAN (4-629): 4.7e-124: 383: 22.17		UB2E3_HUMAN (4-146): 1.24e-74: 223: 64.34
+	Hs
		849480 TRIM1_HUMAN (3-629): 8.74e-115: 361: 20.58		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		175654 TRI18_HUMAN (4-629): 4.7e-124: 383: 22.17		UB2E1_HUMAN (4-146): 4.67e-73: 218: 64.34
+	Hs
		919896 TRIM1_HUMAN (3-629): 8.74e-115: 361: 20.58		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		895945 TRIM1_HUMAN (3-629): 8.74e-115: 361: 20.58		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		175660 TRI18_HUMAN (4-629): 4.7e-124: 383: 22.17		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Hs
		1282363 TRIM1_HUMAN (3-629): 8.74e-115: 361: 20.58		UB2E3_HUMAN (4-146): 1.24e-74: 223: 64.34
+	Hs
		851905 TRIM1_HUMAN (3-629): 8.74e-115: 361: 20.58		UB2E1_HUMAN (4-146): 4.67e-73: 218: 64.34
+	Hs
		896464 Q6GX22_HUMAN (4-629): 2.93e-118: 368: 21.72		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Hs
		896562 TRIM1_HUMAN (3-629): 8.74e-115: 361: 20.58		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53