HitPredict

Interactor 1: BROX (Q5VW32)
BRO1 domain-containing protein BROX (BRO1 domain- and CAAX motif-containing protein)
Gene: BROX

Interactor 2: CHM4B (Q9H444)
Charged multivesicular body protein 4b (Chromatin-modifying protein 4b) (CHMP4b) (SNF7 homolog associated with Alix 1) (SNF7-2) (hSnf7-2) (Vacuolar protein sorting-associated protein 32-2) (Vps32-2) (hVps32-2)
Gene: CHMP4B

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.67	0.60	0.634

Property	Details
Experiments	19403673, 22484091
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation, x-ray crystallography, fluorescence polarization spectroscopy
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005635 - nuclear envelope GO:0070062 - extracellular exosome
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 411 Hs			1 224
+	Hs
		1138944 BROX_HUMAN (1-411): 0: 517: 100.00		CHM4C_HUMAN (1-221): 1.06e-47: 158: 60.78
+	Hs
		1138946 BROX_HUMAN (1-411): 0: 517: 100.00		CHM4A_HUMAN (1-220): 2.41e-43: 147: 56.95
+	Hs
		1138950 BROX_HUMAN (1-411): 0: 517: 100.00		CHMP5_HUMAN (4-219): 7.09e-42: 143: 24.32
+	Hs
		1330347 PTN23_HUMAN (42-397): 4.86e-93: 307: 19.01		CHM4B_HUMAN (1-224): 3.19e-52: 169: 100.00
+	Hs
		1260401 PDC6I_HUMAN (39-392): 2.3e-82: 271: 18.89		CHM4B_HUMAN (1-224): 3.19e-52: 169: 100.00
+	Hs
		1260376 PDC6I_HUMAN (39-392): 2.3e-82: 271: 18.89		CHM4C_HUMAN (1-221): 1.06e-47: 158: 60.78
+	Dm
		1249821 Q9VUH6_DROME (30-392): 5.75e-79: 267: 20.11		CHM4_DROME (1-222): 3.22e-45: 151: 56.28
+	Hs
		1260391 PDC6I_HUMAN (39-392): 2.3e-82: 271: 18.89		CHM4A_HUMAN (1-220): 2.41e-43: 147: 56.95
+	Dm
		1249814 Q9VB05_DROME (34-406): 6.29e-77: 256: 18.64		CHM4_DROME (1-222): 3.22e-45: 151: 56.28
+	Sc
		764903 PALA_YEAST (9-391): 2.88e-72: 240: 17.14		SNF7_YEAST (3-216): 1.69e-34: 124: 38.25
+	Sc
		764862 BRO1_YEAST (39-398): 2.62e-69: 235: 20.32		SNF7_YEAST (3-216): 1.69e-34: 124: 38.25
+	At
		95250 BRO1_ARATH (29-387): 2.91e-66: 227: 18.56		VP322_ARATH (3-224): 1.15e-35: 127: 36.61
+	Sc
		820024 BRO1_YEAST (39-398): 2.62e-69: 235: 20.32		VPS20_YEAST (11-221): 2.51e-32: 118: 20.91
+	At
		95242 BRO1_ARATH (29-387): 2.91e-66: 227: 18.56		VP321_ARATH (3-224): 4.42e-33: 120: 30.49