HitPredict

Interactor 1: M3K5 (Q99683)
Mitogen-activated protein kinase kinase kinase 5 (EC 2.7.11.25) (Apoptosis signal-regulating kinase 1) (ASK-1) (MAPK/ERK kinase kinase 5) (MEK kinase 5) (MEKK 5)
Gene: MAP3K5

Interactor 2: JIP3 (Q9UPT6)
C-Jun-amino-terminal kinase-interacting protein 3 (JIP-3) (JNK-interacting protein 3) (JNK MAP kinase scaffold protein 3) (Mitogen-activated protein kinase 8-interacting protein 3)
Gene: MAPK8IP3

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.64	0.60	0.624

Property	Details
Experiments	12189133, 15258908
Identification Methods	affinity chromatography technology, enzymatic study
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0046330 - positive regulation of JNK cascade
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1374 Hs			1 1336
+	Mm
		192203 M3K5_MOUSE (1-1372): 0: 1451: 94.13		JIP3_MOUSE (1-1336): 0: 954: 93.72
+	Mm
		192189 M3K5_MOUSE (1-1372): 0: 1451: 94.13		JIP4_MOUSE (6-1336): 0: 681: 54.61
+	Hs
		149212 M3K5_HUMAN (1-1374): 0: 1497: 100.00		CTRO_HUMAN (1-425): 1.46e-17: 91.4: 9.17
+	Hs
		1028452 M3K1_HUMAN (686-933): 1.47e-40: 166: 37.31		JIP3_HUMAN (1-1336): 0: 998: 100.00
+	Hs
		229727 M3K3_HUMAN (685-940): 1.87e-43: 171: 40.08		JIP4_HUMAN (6-1336): 0: 671: 54.25
+	Hs
		254476 ROCK2_HUMAN (668-1343): 7.3e-114: 392: 17.55		ROCK1_HUMAN (2-581): 2.57e-21: 103: 9.37
+	Hs
		724727 NEK4_HUMAN (680-1303): 2.63e-113: 378: 19.70		MYH9_HUMAN (228-774): 4.87e-21: 102: 8.04
+	Bt
		1100183 ROCK2_BOVIN (668-1343): 2.49e-113: 391: 17.69		ROCK2_BOVIN (2-348): 4.98e-14: 79.5: 7.48
+	Hs
		254459 KS6A3_HUMAN (623-1074): 1.34e-117: 387: 19.83		ROCK2_HUMAN (2-348): 1.31e-13: 78.3: 7.20
+	Hs
		851635 NEK4_HUMAN (680-1303): 2.63e-113: 378: 19.70		CC136_HUMAN (23-567): 3.52e-09: 63.5: 12.89
+	Hs
		725152 PAK2_HUMAN (686-960): 1.26e-97: 325: 31.27		MYH10_HUMAN (243-769): 2.9e-24: 113: 8.89
+	Hs
		1023100 STK4_HUMAN (686-954): 1.73e-97: 323: 33.95		AKAP9_HUMAN (45-563): 1.97e-11: 71.2: 13.56
+	Hs
		131598 STK25_HUMAN (686-1026): 1.88e-97: 321: 31.29		AKAP9_HUMAN (45-563): 1.97e-11: 71.2: 13.56
+	Hs
		725229 M3K3_HUMAN (685-940): 1.87e-43: 171: 40.08		MYH10_HUMAN (243-769): 2.9e-24: 113: 8.89
+	Hs
		725153 M3K1_HUMAN (686-933): 1.47e-40: 166: 37.31		MYH10_HUMAN (243-769): 2.9e-24: 113: 8.89
+	Hs
		724983 M3K3_HUMAN (685-940): 1.87e-43: 171: 40.08		MYH9_HUMAN (228-774): 4.87e-21: 102: 8.04
+	Hs
		681696 AKT1_HUMAN (438-615): 7.18e-25: 112: 13.64		MYH9_HUMAN (228-774): 4.87e-21: 102: 8.04
+	Hs
		551616 AKT1_HUMAN (438-615): 7.18e-25: 112: 13.64		DESP_HUMAN (27-706): 3.94e-16: 86.8: 11.43
+	Ce
		86713 AKT2_CAEEL (469-597): 3.45e-11: 69.5: 11.28		C6KRI9_CAEEL (50-662): 6.13e-09: 63.1: 12.37
+	Ce
		1095090 AKT2_CAEEL (469-597): 3.45e-11: 69.5: 11.28		Q22847_CAEEL (50-573): 9.67e-09: 62.4: 13.32