HitPredict

Interactor 1: TRIM1 (Q9UJV3)
Probable E3 ubiquitin-protein ligase MID2 (EC 2.3.2.27) (Midin-2) (Midline defect 2) (Midline-2) (RING finger protein 60) (RING-type E3 ubiquitin transferase MID2) (Tripartite motif-containing protein 1)
Gene: MID2

Interactor 2: UB2D4 (Q9Y2X8)
Ubiquitin-conjugating enzyme E2 D4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme D4) (HBUCE1) (Ubiquitin carrier protein D4) (Ubiquitin-protein ligase D4)
Gene: UBE2D4

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.92	1.00	0.964

Property	Details
Experiments	25416956, 21516116, 21143188, 32296183, 19690564, 31515488, unassigned1304
Identification Methods	two hybrid, two hybrid array, validated two hybrid, two hybrid prey pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_UBOX
Common GO terms	GO:0016567 - protein ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 735 Hs			1 147
+	Hs
		849480 TRIM1_HUMAN (1-735): 0: 592: 100.00		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		919896 TRIM1_HUMAN (1-735): 0: 592: 100.00		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		895945 TRIM1_HUMAN (1-735): 0: 592: 100.00		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		1297378 TRIM1_HUMAN (1-735): 0: 592: 100.00		UB2E2_HUMAN (4-146): 3.5e-75: 224: 64.34
+	Hs
		1282363 TRIM1_HUMAN (1-735): 0: 592: 100.00		UB2E3_HUMAN (4-146): 1.24e-74: 223: 64.34
+	Hs
		851905 TRIM1_HUMAN (1-735): 0: 592: 100.00		UB2E1_HUMAN (4-146): 4.67e-73: 218: 64.34
+	Hs
		896562 TRIM1_HUMAN (1-735): 0: 592: 100.00		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Hs
		1151762 Q6GX22_HUMAN (21-735): 0: 550: 95.66		UB2D4_HUMAN (1-147): 8.89e-84: 243: 100.00
+	Hs
		175730 TRI18_HUMAN (21-717): 8.76e-175: 518: 72.60		UB2D4_HUMAN (1-147): 8.89e-84: 243: 100.00
+	Hs
		175653 TRI18_HUMAN (21-717): 8.76e-175: 518: 72.60		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		175664 TRI18_HUMAN (21-717): 8.76e-175: 518: 72.60		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		896464 Q6GX22_HUMAN (21-735): 0: 550: 95.66		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Hs
		175658 TRI18_HUMAN (21-717): 8.76e-175: 518: 72.60		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		175710 TRI18_HUMAN (21-717): 8.76e-175: 518: 72.60		UB2E2_HUMAN (4-146): 3.5e-75: 224: 64.34
+	Hs
		175703 TRI18_HUMAN (21-717): 8.76e-175: 518: 72.60		UB2E3_HUMAN (4-146): 1.24e-74: 223: 64.34
+	Hs
		175654 TRI18_HUMAN (21-717): 8.76e-175: 518: 72.60		UB2E1_HUMAN (4-146): 4.67e-73: 218: 64.34
+	Hs
		175660 TRI18_HUMAN (21-717): 8.76e-175: 518: 72.60		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Hs
		919872 TRI36_HUMAN (19-718): 1.62e-98: 322: 21.73		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		849330 TRI25_HUMAN (24-706): 9.41e-99: 319: 20.96		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		895794 TRI25_HUMAN (24-706): 9.41e-99: 319: 20.96		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84