HitPredict

Interactor 1: NHRF1 (O14745)
Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (NHERF-1) (Ezrin-radixin-moesin-binding phosphoprotein 50) (EBP50) (Regulatory cofactor of Na(+)/H(+) exchanger) (Sodium-hydrogen exchanger regulatory factor 1) (Solute carrier family 9 isoform A3 regulatory factor 1)
Gene: NHERF1

Interactor 2: CFTR (P13569)
Cystic fibrosis transmembrane conductance regulator (CFTR) (ATP-binding cassette sub-family C member 7) (Channel conductance-controlling ATPase) (EC 5.6.1.6) (cAMP-dependent chloride channel)
Gene: CFTR

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.97	0.60	0.765

Property	Details
Experiments	9613608, 10852925, 12471024, 9677412, 12403779, 26618866, 12615054, 9671706, 31324722, 17110338, 19446522, 18754678, 28360110, 17244609
Identification Methods	pull down, affinity chromatography technology, unspecified method, surface plasmon resonance, fluorescence polarization spectroscopy, isothermal titration calorimetry, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, cross-linking study, filter binding
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0016020 - membrane GO:0016324 - apical plasma membrane GO:0017081 - chloride channel regulator activity GO:0030165 - PDZ domain binding
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 358 Hs			1 1480
+	Hs
		529166 NHRF2_HUMAN (5-358): 2.98e-67: 216: 49.44		CFTR_HUMAN (1-1480): 0: 1031: 100.00
+	Mm
		641306 NHRF1_MOUSE (1-358): 4.9e-77: 242: 83.89		CFTR_MOUSE (1-1468): 0: 957: 78.29
+	Hs
		529099 SCRIB_HUMAN (7-337): 1.45e-29: 122: 19.83		CFTR_HUMAN (1-1480): 0: 1031: 100.00
+	Hs
		529235 NHRF3_HUMAN (1-133): 3.86e-21: 96.3: 32.35		CFTR_HUMAN (1-1480): 0: 1031: 100.00
+	Hs
		529050 DLG1_HUMAN (55-238): 9.08e-13: 71.6: 18.85		CFTR_HUMAN (1-1480): 0: 1031: 100.00
+	Hs
		529355 NHRF4_HUMAN (93-235): 8.62e-12: 68.1: 22.22		CFTR_HUMAN (1-1480): 0: 1031: 100.00
+	Hs
		529578 SNX27_HUMAN (12-91): 4.35e-09: 59.7: 37.63		CFTR_HUMAN (1-1480): 0: 1031: 100.00
+	Hs
		153846 NHRF1_HUMAN (1-358): 2.51e-82: 256: 100.00		MRP4_HUMAN (5-647): 2.25e-59: 227: 15.67
+	Hs
		153939 NHRF1_HUMAN (1-358): 2.51e-82: 256: 100.00		MRP2_HUMAN (798-1466): 4.31e-56: 217: 15.74
+	Rn
		1144126 NHRF1_RAT (1-358): 2.87e-79: 248: 86.35		MRP2_RAT (773-1466): 1.07e-55: 215: 15.08
+	Hs
		179358 NHRF2_HUMAN (5-358): 2.98e-67: 216: 49.44		MRP4_HUMAN (5-647): 2.25e-59: 227: 15.67
+	Hs
		179356 SCRIB_HUMAN (7-337): 1.45e-29: 122: 19.83		MRP4_HUMAN (5-647): 2.25e-59: 227: 15.67
+	Hs
		179363 NHRF3_HUMAN (1-133): 3.86e-21: 96.3: 32.35		MRP4_HUMAN (5-647): 2.25e-59: 227: 15.67
+	Hs
		1132912 NHRF3_HUMAN (1-133): 3.86e-21: 96.3: 32.35		MRP2_HUMAN (798-1466): 4.31e-56: 217: 15.74
+	Hs
		67899 PDZ1P_HUMAN (142-346): 3.81e-15: 77.8: 19.02		MRP4_HUMAN (5-647): 2.25e-59: 227: 15.67
+	Hs
		179374 SNX27_HUMAN (12-91): 4.35e-09: 59.7: 37.63		MRP4_HUMAN (5-647): 2.25e-59: 227: 15.67
+	Hs
		1200079 NHRF4_HUMAN (93-235): 8.62e-12: 68.1: 22.22		MRP2_HUMAN (798-1466): 4.31e-56: 217: 15.74