HitPredict

Interactor 1: UB2L6 (O14933)
Ubiquitin/ISG15-conjugating enzyme E2 L6 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme L6) (Retinoic acid-induced gene B protein) (RIG-B) (UbcH8) (Ubiquitin carrier protein L6) (Ubiquitin-protein ligase L6)
Gene: UBE2L6

Interactor 2: RNF25 (Q96BH1)
E3 ubiquitin-protein ligase RNF25 (EC 2.3.2.27) (RING finger protein 25) (RING finger protein AO7)
Gene: RNF25

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.48	1.00	0.698

Property	Details
Experiments	19690564
Identification Methods	two hybrid, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	RWD~UQ_con, UQ_con~zf-RING_2
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0005634 - nucleus GO:0005829 - cytosol GO:0006511 - ubiquitin-dependent protein catabolic process
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 153 Hs			1 459
+	Mm
		919926 UB2D2_MOUSE (2-149): 3.7e-75: 222: 37.58		RNF25_MOUSE (1-459): 0: 669: 82.35
+	Mm
		854556 UB2E3_MOUSE (3-148): 1.55e-68: 207: 31.97		RNF25_MOUSE (1-459): 0: 669: 82.35
+	Hs
		1285495 UB2D4_HUMAN (2-149): 5.5e-76: 224: 38.93		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Hs
		849398 UB2D1_HUMAN (2-149): 1.04e-75: 223: 38.93		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Mm
		854550 UB2E1_MOUSE (3-148): 2.68e-67: 204: 34.01		RNF25_MOUSE (1-459): 0: 669: 82.35
+	Hs
		919815 UB2D2_HUMAN (2-149): 3.7e-75: 222: 37.58		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Hs
		895869 UB2D3_HUMAN (2-149): 5.99e-74: 219: 36.24		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Mm
		932609 UB2L3_MOUSE (1-152): 6.11e-66: 199: 55.26		RNF25_MOUSE (1-459): 0: 669: 82.35
+	Hs
		1285490 UB2E2_HUMAN (3-148): 7.71e-69: 208: 32.65		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Hs
		1282323 UB2E3_HUMAN (3-148): 1.55e-68: 207: 31.97		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Hs
		851863 UB2E1_HUMAN (3-148): 5.11e-67: 203: 34.01		RNF25_HUMAN (1-459): 0: 651: 100.00
+	Dm
		54265 UBCD1_DROME (2-149): 1.11e-74: 221: 37.58		A1Z9I6_DROME (15-257): 1.93e-84: 265: 35.92
+	Dm
		54267 UBCD2_DROME (3-148): 4.79e-69: 209: 34.69		A1Z9I6_DROME (15-257): 1.93e-84: 265: 35.92
+	At
		1118123 UBC29_ARATH (2-149): 1.54e-72: 215: 33.56		Q4TU20_ARATH (23-277): 3.55e-80: 254: 25.09
+	At
		1258961 UBC29_ARATH (2-149): 1.54e-72: 215: 33.56		Q8W4A3_ARATH (128-277): 2.36e-44: 157: 24.16
+	Zm
		41536 B4FC97_MAIZE (2-149): 4.98e-76: 224: 36.24		A0A1D6NML1_MAIZE (33-127): 4.84e-25: 107: 25.00