HitPredict

Interactor 1: ML12B (O14950)
Myosin regulatory light chain 12B (MLC-2A) (MLC-2) (Myosin regulatory light chain 2-B, smooth muscle isoform) (Myosin regulatory light chain 20 kDa) (MLC20) (Myosin regulatory light chain MRLC2) (SHUJUN-1)
Gene: MYL12B

Interactor 2: MYL6 (P60660)
Myosin light polypeptide 6 (17 kDa myosin light chain) (LC17) (Myosin light chain 3) (MLC-3) (Myosin light chain alkali 3) (Myosin light chain A3) (Smooth muscle and nonmuscle myosin light chain alkali 6)
Gene: MYL6

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	0.60	0.667

Property	Details
Experiments	24981860, 33961781, 35271311
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005509 - calcium ion binding GO:0005829 - cytosol GO:0005903 - brush border GO:0016460 - myosin II complex GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 172 Hs			1 151
+	Hs
		409446 ML12A_HUMAN (15-172): 2.3e-65: 199: 99.37		MYL1_HUMAN (3-150): 1.84e-56: 176: 74.32
+	Hs
		159536 ML12B_HUMAN (1-172): 1.42e-67: 204: 100.00		MYL6B_HUMAN (3-150): 2.63e-42: 141: 81.76
+	Hs
		538639 MYL9_HUMAN (1-172): 2.61e-66: 201: 93.02		MYL6B_HUMAN (3-150): 2.63e-42: 141: 81.76
+	Hs
		538637 ML12A_HUMAN (15-172): 2.3e-65: 199: 99.37		MYL6B_HUMAN (3-150): 2.63e-42: 141: 81.76
+	Hs
		624346 MYL9_HUMAN (1-172): 2.61e-66: 201: 93.02		MYL6_HUMAN (1-151): 9.61e-42: 137: 100.00
+	Hs
		580632 ML12A_HUMAN (15-172): 2.3e-65: 199: 99.37		MYL6_HUMAN (1-151): 9.61e-42: 137: 100.00
+	Ce
		585992 MLR1_CAEEL (24-170): 2.51e-39: 133: 49.32		MLE_CAEEL (12-151): 6.35e-36: 123: 46.43
+	Ce
		585996 MLR2_CAEEL (24-170): 1.91e-39: 133: 49.32		MLE_CAEEL (12-151): 6.35e-36: 123: 46.43
+	At
		483086 CDPK3_ARATH (103-172): 4.65e-14: 70.6: 22.86		CALM4_ARATH (5-77): 2.69e-11: 59.4: 27.40
+	At
		886549 CDPK3_ARATH (103-172): 4.65e-14: 70.6: 22.86		CALM7_ARATH (5-77): 2.96e-11: 59.4: 27.40
+	At
		976625 CDPK3_ARATH (103-172): 4.65e-14: 70.6: 22.86		CALM6_ARATH (5-77): 5.06e-11: 58.6: 26.03
+	At
		989458 CDPK1_ARATH (62-172): 3.22e-14: 71: 20.18		CDPK1_ARATH (73-150): 1e-09: 57.5: 21.52
+	At
		483177 CDPKU_ARATH (87-172): 5.02e-13: 67.6: 29.07		CALM4_ARATH (5-77): 2.69e-11: 59.4: 27.40
+	At
		886638 CDPKU_ARATH (87-172): 5.02e-13: 67.6: 29.07		CALM7_ARATH (5-77): 2.96e-11: 59.4: 27.40
+	At
		976699 CDPKU_ARATH (87-172): 5.02e-13: 67.6: 29.07		CALM6_ARATH (5-77): 5.06e-11: 58.6: 26.03
+	At
		1104924 CDPK4_ARATH (37-172): 5.35e-13: 67.6: 19.15		CDPK4_ARATH (73-150): 3.27e-09: 56: 22.79
+	At
		1105900 CDPKB_ARATH (103-172): 1.13e-12: 66.8: 27.14		CDPKB_ARATH (73-150): 4.48e-09: 55.6: 22.79
+	At
		886540 CALM6_ARATH (32-93): 7.82e-12: 61.7: 31.82		CALM7_ARATH (5-77): 2.96e-11: 59.4: 27.40
+	Hs
		483903 CALL3_HUMAN (32-93): 8.36e-11: 58.6: 25.76		CALM1_HUMAN (5-77): 1.13e-11: 60.6: 28.77
+	Hs
		483976 CALL3_HUMAN (32-93): 8.36e-11: 58.6: 25.76		CALM2_HUMAN (5-77): 1.13e-11: 60.6: 28.77