HitPredict

Interactor 1: SMAD7 (O15105)
Mothers against decapentaplegic homolog 7 (MAD homolog 7) (Mothers against DPP homolog 7) (Mothers against decapentaplegic homolog 8) (MAD homolog 8) (Mothers against DPP homolog 8) (SMAD family member 7) (SMAD 7) (Smad7) (hSMAD7)
Gene: SMAD7

Interactor 2: ITCH (Q96J02)
E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
Gene: ITCH

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.79	0.60	0.691

Property	Details
Experiments	28400336, 15946939, 33961781, 22773947
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0032991 - protein-containing complex
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 426 Hs			1 903
+	Hs
		206970 SMAD6_HUMAN (1-425): 5.75e-147: 428: 43.56		ITCH_HUMAN (1-903): 0: 926: 100.00
+	Hs
		167393 SMAD7_HUMAN (1-426): 1.58e-170: 486: 100.00		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		125003 SMAD7_HUMAN (1-426): 1.58e-170: 486: 100.00		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		206975 SMAD6_HUMAN (1-425): 5.75e-147: 428: 43.56		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		1305881 SMAD5_HUMAN (79-424): 5.54e-146: 425: 24.08		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		167385 SMAD7_HUMAN (1-426): 1.58e-170: 486: 100.00		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Hs
		125023 SMAD6_HUMAN (1-425): 5.75e-147: 428: 43.56		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Dm
		785516 MAD_DROME (72-424): 2.77e-151: 438: 25.48		SUDX_DROME (19-903): 0: 788: 48.29
+	Hs
		206971 SMAD6_HUMAN (1-425): 5.75e-147: 428: 43.56		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Hs
		167342 SMAD7_HUMAN (1-426): 1.58e-170: 486: 100.00		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		206944 SMAD6_HUMAN (1-425): 5.75e-147: 428: 43.56		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		808240 SMAD5_HUMAN (79-424): 5.54e-146: 425: 24.08		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Mm
		56823 SMAD7_MOUSE (1-426): 2.83e-174: 495: 98.12		SMUF2_MOUSE (1-358): 2.99e-46: 179: 16.67
+	Hs
		167395 SMAD7_HUMAN (1-426): 1.58e-170: 486: 100.00		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		167394 SMAD7_HUMAN (1-426): 1.58e-170: 486: 100.00		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Mm
		192495 SMAD7_MOUSE (1-426): 2.83e-174: 495: 98.12		SMUF1_MOUSE (1-535): 3.62e-41: 164: 14.07
+	Hs
		206977 SMAD6_HUMAN (1-425): 5.75e-147: 428: 43.56		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		206976 SMAD6_HUMAN (1-425): 5.75e-147: 428: 43.56		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Hs
		1305886 SMAD5_HUMAN (79-424): 5.54e-146: 425: 24.08		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Dm
		785477 MAD_DROME (72-424): 2.77e-151: 438: 25.48		SMUF1_DROME (16-518): 1.15e-39: 161: 16.19