HitPredict

Interactor 1: TRI18 (O15344)
E3 ubiquitin-protein ligase Midline-1 (EC 2.3.2.27) (Midin) (Putative transcription factor XPRF) (RING finger protein 59) (RING finger protein Midline-1) (RING-type E3 ubiquitin transferase Midline-1) (Tripartite motif-containing protein 18)
Gene: MID1

Interactor 2: UB2D4 (Q9Y2X8)
Ubiquitin-conjugating enzyme E2 D4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme D4) (HBUCE1) (Ubiquitin carrier protein D4) (Ubiquitin-protein ligase D4)
Gene: UBE2D4

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.80	1.00	0.896

Property	Details
Experiments	32296183, 19549727, 21143188, unassigned1304
Identification Methods	two hybrid, validated two hybrid, two hybrid array, two hybrid prey pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_UBOX
Common GO terms	GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 667 Hs			1 147
+	Hs
		175653 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		175664 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		175658 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		175710 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2E2_HUMAN (4-146): 3.5e-75: 224: 64.34
+	Hs
		175703 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2E3_HUMAN (4-146): 1.24e-74: 223: 64.34
+	Hs
		1151762 Q6GX22_HUMAN (1-665): 1.26e-170: 505: 76.24		UB2D4_HUMAN (1-147): 8.89e-84: 243: 100.00
+	Hs
		175654 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UB2E1_HUMAN (4-146): 4.67e-73: 218: 64.34
+	Hs
		175660 TRI18_HUMAN (1-667): 1.27e-180: 530: 100.00		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Hs
		1356055 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2D4_HUMAN (1-147): 8.89e-84: 243: 100.00
+	Hs
		849480 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		919896 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		895945 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		1297378 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2E2_HUMAN (4-146): 3.5e-75: 224: 64.34
+	Hs
		1282363 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2E3_HUMAN (4-146): 1.24e-74: 223: 64.34
+	Hs
		896464 Q6GX22_HUMAN (1-665): 1.26e-170: 505: 76.24		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Hs
		851905 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UB2E1_HUMAN (4-146): 4.67e-73: 218: 64.34
+	Hs
		896562 TRIM1_HUMAN (1-665): 4.96e-164: 490: 72.81		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Hs
		849330 TRI25_HUMAN (4-656): 9.25e-108: 341: 22.80		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		895794 TRI25_HUMAN (4-656): 9.25e-108: 341: 22.80		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		896442 TRI25_HUMAN (4-656): 9.25e-108: 341: 22.80		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53