HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: EGFR (P00533)
Epidermal growth factor receptor (EC 2.7.10.1) (Proto-oncogene c-ErbB-1) (Receptor tyrosine-protein kinase erbB-1)
Gene: EGFR

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.92	1.00	0.961

Property	Details
Experiments	25402006, 24658140, 22973453, 9737977, 10026169, 9843575, unassigned2173
Identification Methods	two hybrid, protein complementation assay, affinity chromatography technology, pull down, ubiquitin reconstruction
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	PK_Tyr_Ser-Thr~SH2, PK_Tyr_Ser-Thr~SH3_1, PK_Tyr_Ser-Thr~SH3_9
Common GO terms	GO:0005737 - cytoplasm GO:0007165 - signal transduction GO:0007173 - epidermal growth factor receptor signaling pathway GO:0045944 - positive regulation of transcription by RNA polymerase II
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 1210
+	Hs
		337883 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		EGFR_HUMAN (1-1210): 0: 1241: 100.00
+	Hs
		209056 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ERBB4_HUMAN (1-1199): 0: 1137: 49.46
+	Hs
		208972 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ERBB2_HUMAN (5-1200): 0: 1111: 48.49
+	Hs
		383587 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ERBB2_HUMAN (5-1200): 0: 1111: 48.49
+	Hs
		208992 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ERBB3_HUMAN (1-1210): 0: 1080: 40.95
+	Hs
		557604 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ERBB3_HUMAN (1-1210): 0: 1080: 40.95
+	Hs
		208976 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		RET_HUMAN (420-1058): 5.61e-129: 425: 22.69
+	Hs
		434130 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		RET_HUMAN (420-1058): 5.61e-129: 425: 22.69
+	Hs
		209018 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		EPHA4_HUMAN (660-1016): 3.35e-120: 398: 31.52
+	Hs
		208996 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		EPHA2_HUMAN (665-1016): 5.77e-117: 389: 32.79
+	Hs
		557644 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		EPHB1_HUMAN (640-1071): 8.83e-123: 405: 28.06
+	Rn
		88904 D4A3M8_RAT (1-380): 3.21e-98: 298: 95.79		INSR_RAT (579-1032): 2.5e-106: 367: 23.66
+	Hs
		209039 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ACK1_HUMAN (691-967): 4.54e-56: 214: 39.58
+	Hs
		209036 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		FAK1_HUMAN (716-991): 1.58e-53: 206: 37.68
+	Hs
		209001 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ABL2_HUMAN (707-974): 5.53e-52: 202: 40.37
+	Mm
		222878 NCK1_MOUSE (1-379): 6.79e-91: 279: 68.24		ACK1_MOUSE (691-967): 2.4e-53: 206: 38.87
+	Hs
		557666 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		FAK1_HUMAN (716-991): 1.58e-53: 206: 37.68
+	Hs
		557630 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ABL2_HUMAN (707-974): 5.53e-52: 202: 40.37
+	Hs
		557633 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		KSYK_HUMAN (716-961): 1.13e-53: 201: 41.70
+	Hs
		435526 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		IGF1R_HUMAN (354-658): 1.12e-14: 81.4: 18.47