HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: WASF1 (Q92558)
Actin-binding protein WASF1 (Protein WAVE-1) (Verprolin homology domain-containing protein 1) (Wiskott-Aldrich syndrome protein family member 1) (WASP family protein member 1)
Gene: WASF1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.85	0.60	0.717

Property	Details
Experiments	26186194, 28514442, 32296183, 33961781, unassigned1304
Identification Methods	affinity chromatography technology, two hybrid, anti tag coimmunoprecipitation, two hybrid array, validated two hybrid, two hybrid prey pooling approach
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 559
+	Hs
		557755 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		WASF1_HUMAN (1-559): 2.63e-73: 245: 100.00
+	Hs
		209188 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		WASF3_HUMAN (1-557): 1.12e-43: 164: 45.73
+	Hs
		209196 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		WASF2_HUMAN (1-541): 2.37e-42: 160: 42.14
+	Hs
		557843 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		WASF2_HUMAN (1-541): 2.37e-42: 160: 42.14
+	Hs
		209039 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ACK1_HUMAN (148-530): 2.56e-25: 112: 19.49
+	Hs
		166030 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		SYNJ2_HUMAN (46-507): 2.06e-31: 132: 13.92
+	Hs
		557670 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ACK1_HUMAN (148-530): 2.56e-25: 112: 19.49
+	Mm
		222878 NCK1_MOUSE (1-379): 6.79e-91: 279: 68.24		ACK1_MOUSE (162-530): 2.06e-21: 100: 18.09
+	Hs
		655430 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		WASF1_HUMAN (1-559): 2.63e-73: 245: 100.00
+	Hs
		203208 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		SYNJ1_HUMAN (41-523): 2.02e-11: 69: 12.53
+	Mm
		643108 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		WASF1_MOUSE (1-559): 8.6e-72: 241: 98.39
+	Ce
		712432 Q95PW9_CAEEL (1-379): 5.2e-75: 239: 37.88		SC161_CAEEL (208-492): 3.01e-09: 62: 14.97
+	Hs
		1349289 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		WASF2_HUMAN (1-541): 2.37e-42: 160: 42.14
+	Hs
		655509 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		WASF3_HUMAN (1-557): 1.12e-43: 164: 45.73
+	Hs
		166056 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		SYNJ2_HUMAN (46-507): 2.06e-31: 132: 13.92
+	Hs
		782781 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		ACK1_HUMAN (148-530): 2.56e-25: 112: 19.49
+	Hs
		166032 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		SYNJ2_HUMAN (46-507): 2.06e-31: 132: 13.92
+	Hs
		655342 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		EP300_HUMAN (77-554): 1.1e-28: 124: 14.91
+	Mm
		70897 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		EP300_MOUSE (77-554): 6.02e-27: 118: 13.31
+	Hs
		655334 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		ACK1_HUMAN (148-530): 2.56e-25: 112: 19.49