HitPredict

Interactor 1: PRKN (O60260)
E3 ubiquitin-protein ligase parkin (Parkin) (EC 2.3.2.31) (Parkin RBR E3 ubiquitin-protein ligase) (Parkinson juvenile disease protein 2) (Parkinson disease protein 2)
Gene: PRKN

Interactor 2: STAM2 (O75886)
Signal transducing adapter molecule 2 (STAM-2) (Hrs-binding protein)
Gene: STAM2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.70	1.00	0.839

Property	Details
Experiments	35509820, 35256949, 32814053
Identification Methods	affinity chromatography technology, two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	SH3_1~ubiquitin, UIM~ubiquitin, VHS~ubiquitin
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0016236 - macroautophagy GO:0043130 - ubiquitin binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 465 Hs			1 525
+	Hs
		226013 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		TOM1_HUMAN (1-444): 1.01e-80: 262: 17.68
+	Hs
		276992 UBB_HUMAN (1-234): 1.14e-59: 197: 17.92		STAM2_HUMAN (1-525): 4.7e-141: 418: 100.00
+	Hs
		227403 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		SPTN1_HUMAN (103-481): 2.5e-55: 202: 14.59
+	Hs
		276993 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		STAM2_HUMAN (1-525): 4.7e-141: 418: 100.00
+	Hs
		159798 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		HGS_HUMAN (135-518): 7.87e-15: 79.4: 10.95
+	Hs
		227109 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		GRB2_HUMAN (207-262): 4.5e-12: 67.4: 52.63
+	Hs
		3258 HOIL1_HUMAN (12-448): 5.59e-68: 227: 16.36		A0A0S2Z4Q4_HUMAN (8-518): 9.26e-71: 241: 14.13
+	Hs
		475230 UBB_HUMAN (1-234): 1.14e-59: 197: 17.92		GGA1_HUMAN (2-516): 8.21e-63: 218: 14.50
+	Sc
		477314 UBI4P_YEAST (1-229): 3.48e-61: 205: 18.72		VPS27_YEAST (1-488): 4.18e-59: 208: 16.02
+	Sc
		477454 UBI4P_YEAST (1-229): 3.48e-61: 205: 18.72		GGA1_YEAST (2-500): 4.76e-55: 196: 14.70
+	Hs
		475215 UBB_HUMAN (1-234): 1.14e-59: 197: 17.92		GGA3_HUMAN (1-510): 1.35e-56: 203: 15.73
+	Sc
		477270 UBI4P_YEAST (1-229): 3.48e-61: 205: 18.72		GGA2_YEAST (2-426): 2.15e-54: 194: 14.35
+	Sc
		477263 UBI4P_YEAST (1-229): 3.48e-61: 205: 18.72		HSE1_YEAST (15-449): 6.92e-53: 187: 19.56
+	Hs
		241137 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		TOM1_HUMAN (1-444): 1.01e-80: 262: 17.68
+	Bt
		477668 UBC_BOVIN (1-149): 6.08e-49: 179: 19.33		Q58CQ6_BOVIN (2-422): 1.22e-59: 205: 13.66
+	Hs
		272789 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		TM1L1_HUMAN (6-494): 3.07e-63: 215: 16.02
+	Hs
		160178 HOIL1_HUMAN (12-448): 5.59e-68: 227: 16.36		HGS_HUMAN (135-518): 7.87e-15: 79.4: 10.95
+	Hs
		474986 UBB_HUMAN (1-234): 1.14e-59: 197: 17.92		GRB2_HUMAN (207-262): 4.5e-12: 67.4: 52.63
+	Hs
		159860 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		HGS_HUMAN (135-518): 7.87e-15: 79.4: 10.95
+	Hs
		475592 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		GRB2_HUMAN (207-262): 4.5e-12: 67.4: 52.63