HitPredict

Interactor 1: PRKN (O60260)
E3 ubiquitin-protein ligase parkin (Parkin) (EC 2.3.2.31) (Parkin RBR E3 ubiquitin-protein ligase) (Parkinson juvenile disease protein 2) (Parkinson disease protein 2)
Gene: PRKN

Interactor 2: HSP74 (P34932)
Heat shock 70 kDa protein 4 (HSP70RY) (Heat shock 70-related protein APG-2) (Heat shock protein family H member 2)
Gene: HSPA4

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	0.60	0.751

Property	Details
Experiments	18248624, 15603737, 29180628, 25959826, 35256949, 16714300, 12676955, 18784277, 14532270
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005739 - mitochondrion GO:0005829 - cytosol
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 465 Hs			1 840
+	Hs
		226163 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		HS74L_HUMAN (1-840): 0: 959: 63.21
+	Hs
		228229 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		HS105_HUMAN (1-840): 0: 938: 62.95
+	At
		1092265 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		HSP7O_ARATH (1-832): 0: 877: 39.19
+	At
		94958 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		HSP7P_ARATH (1-832): 0: 870: 39.19
+	Hs
		226353 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		HSP7C_HUMAN (564-692): 5.97e-20: 97: 16.92
+	Hs
		226336 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		HS71A_HUMAN (571-686): 1.59e-19: 95.5: 23.93
+	Hs
		226663 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		HS71L_HUMAN (564-691): 7.22e-19: 93.5: 22.48
+	Hs
		226452 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		HSP76_HUMAN (587-690): 1.94e-16: 85.8: 22.86
+	Mm
		1143065 PRKN_MOUSE (1-465): 6.53e-131: 388: 83.66		HS71A_MOUSE (571-686): 1e-17: 89.7: 23.08
+	At
		1092279 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		HSP7E_ARATH (4-585): 4.1e-86: 289: 34.34
+	Hs
		312461 ARI2_HUMAN (222-464): 1.56e-64: 217: 20.82		HSP7C_HUMAN (564-692): 5.97e-20: 97: 16.92
+	Hs
		312496 ARI2_HUMAN (222-464): 1.56e-64: 217: 20.82		HS71L_HUMAN (564-691): 7.22e-19: 93.5: 22.48
+	Hs
		474905 UBB_HUMAN (1-234): 1.14e-59: 197: 17.92		HSP7C_HUMAN (564-692): 5.97e-20: 97: 16.92
+	Hs
		475281 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		HSP7C_HUMAN (564-692): 5.97e-20: 97: 16.92