HitPredict

Interactor 1: PRKN (O60260)
E3 ubiquitin-protein ligase parkin (Parkin) (EC 2.3.2.31) (Parkin RBR E3 ubiquitin-protein ligase) (Parkinson juvenile disease protein 2) (Parkinson disease protein 2)
Gene: PRKN

Interactor 2: SIM2 (Q14190)
Single-minded homolog 2 (Class E basic helix-loop-helix protein 15) (bHLHe15)
Gene: SIM2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.60	0.60	0.601

Property	Details
Experiments	15963499, 32814053
Identification Methods	affinity chromatography technology, two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000122 - negative regulation of transcription by RNA polymerase II
Homologous interactions	8

Homologous interactions

Color coding for hit scores

	1 465 Hs			1 667
+	Hs
		227152 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		SIM1_HUMAN (1-663): 2.16e-160: 481: 55.31
+	Hs
		227589 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		HIF1A_HUMAN (2-501): 2.75e-126: 395: 32.11
+	Hs
		1050595 ARI1_HUMAN (163-464): 3.95e-74: 244: 19.67		SIM2_HUMAN (1-667): 0: 541: 100.00
+	Mm
		1337460 RNF14_MOUSE (161-464): 6.04e-70: 231: 20.63		BMAL1_MOUSE (2-496): 7.69e-116: 362: 20.97
+	Hs
		1317084 HOIL1_HUMAN (12-448): 5.59e-68: 227: 16.36		ARNT2_HUMAN (2-612): 6.83e-112: 354: 18.90
+	Hs
		476267 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		EPAS1_HUMAN (2-667): 1.56e-133: 415: 23.86
+	Hs
		475831 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		HIF1A_HUMAN (2-501): 2.75e-126: 395: 32.11
+	Dm
		247700 Q9W418_DROME (1-77): 5.42e-30: 124: 32.47		CYCL_DROME (2-332): 3.27e-108: 335: 25.00