HitPredict

Interactor 1: PIF3 (O80536)
Transcription factor PIF3 (Basic helix-loop-helix protein 8) (AtbHLH8) (bHLH 8) (Phytochrome-associated protein 3) (Phytochrome-interacting factor 3) (Transcription factor EN 100) (bHLH transcription factor bHLH008)
Gene: PIF3

Interactor 2: PHYA (P14712)
Phytochrome A (Protein ELONGATED HYPOCOTYL 8) (Protein FAR RED ELONGATED 1) (Protein FAR RED ELONGATED HYPOCOTYL 2)
Gene: PHYA

Species: Arabidopsis thaliana

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.96	1.00	0.981

Property	Details
Experiments	19286967, 19208901, 11069292, 10995393, 9845368, 15486102, 12006496, 23746445, 17827270, 16885032, 16705748, 15448264
Identification Methods	affinity chromatography technology, pull down, two hybrid, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	HLH~PAS
Common GO terms	GO:0005634 - nucleus GO:0006355 - regulation of DNA-templated transcription GO:0009409 - response to cold
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 524 At			1 1122
+	At
		285436 PIF3_ARATH (1-524): 4.1e-105: 326: 100.00		PHYB_ARATH (3-1122): 0: 1202: 51.68
+	At
		285438 PIF3_ARATH (1-524): 4.1e-105: 326: 100.00		PHYD_ARATH (2-1120): 0: 1179: 51.73
+	At
		285437 PIF3_ARATH (1-524): 4.1e-105: 326: 100.00		PHYC_ARATH (6-1118): 0: 1111: 51.61
+	At
		539631 PIF1_ARATH (90-500): 7.04e-36: 141: 21.90		PHYA_ARATH (1-1122): 0: 1205: 100.00
+	At
		539627 PIF5_ARATH (221-475): 1.78e-35: 139: 29.32		PHYA_ARATH (1-1122): 0: 1205: 100.00
+	At
		539634 PIF4_ARATH (10-516): 2.18e-35: 139: 22.09		PHYA_ARATH (1-1122): 0: 1205: 100.00
+	At
		539668 PIF1_ARATH (90-500): 7.04e-36: 141: 21.90		PHYB_ARATH (3-1122): 0: 1202: 51.68
+	At
		539664 PIF5_ARATH (221-475): 1.78e-35: 139: 29.32		PHYB_ARATH (3-1122): 0: 1202: 51.68
+	At
		539673 PIF4_ARATH (10-516): 2.18e-35: 139: 22.09		PHYB_ARATH (3-1122): 0: 1202: 51.68
+	At
		539670 PIL1_ARATH (197-501): 2.4e-35: 138: 24.10		PHYB_ARATH (3-1122): 0: 1202: 51.68
+	At
		539669 PIF6_ARATH (218-505): 7.4e-35: 136: 23.81		PHYB_ARATH (3-1122): 0: 1202: 51.68
+	At
		539635 HFR1_ARATH (302-458): 6.38e-31: 123: 35.85		PHYA_ARATH (1-1122): 0: 1205: 100.00
+	At
		539676 HFR1_ARATH (302-458): 6.38e-31: 123: 35.85		PHYB_ARATH (3-1122): 0: 1202: 51.68
+	At
		539661 PIF7_ARATH (217-418): 1.32e-27: 115: 32.37		PHYB_ARATH (3-1122): 0: 1202: 51.68