HitPredict

Interactor 1: RPOD (P00579)
RNA polymerase sigma factor RpoD (Sigma-70)
Gene: RPOD

Interactor 2: RPOB (P0A8V2)
DNA-directed RNA polymerase subunit beta (RNAP subunit beta) (EC 2.7.7.6) (RNA polymerase subunit beta) (Transcriptase subunit beta)
Gene: RPOB

Species: Escherichia coli K-12

Source: intact

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.79 1.00 0.892

The method score is based on:

Property Details
Experiments 16606699, 19366670, 22135460, 15690043
Identification Methods affinity chromatography technology, lambda repressor two hybrid, tandem affinity purification, pull down
Interaction Type physical association, association

The annotation score is based on:

Property Details
Interacting Pfam domains RNA_pol_Rpb2_6~Sigma70_ner, RNA_pol_Rpb2_1~Sigma70_r1_1, RNA_pol_Rpb2_1~Sigma70_r1_2, RNA_pol_Rpb2_1~Sigma70_r2, RNA_pol_Rpb2_6~Sigma70_r2, RNA_pol_Rpb2_7~Sigma70_r2, RNA_pol_Rpb2_1~Sigma70_r3, RNA_pol_Rpb2_6~Sigma70_r3, RNA_pol_Rpb2_7~Sigma70_r3, RNA_pol_Rpb2_6~Sigma70_r4
Common GO terms GO:0000345 - cytosolic DNA-directed RNA polymerase complex
GO:0003677 - DNA binding
GO:0005829 - cytosol
GO:0006352 - DNA-templated transcription initiation
GO:0009408 - response to heat
and 1 others

more...
Homologous interactions 12

Homologous interactions

Color coding for hit scores

1 613
Ec

1 1342
+ Nv
+ Hp
+ Ec
+ Ec
+ Ec
+ Hp
+ Mp
+ Bs
+ Ss
+ Ss
+ Ss
+ Bs

Service provided by Combinatics, Japan

Last updated: 15 Jul 2024