HitPredict

Interactor 1: A1AT (P01009)
Alpha-1-antitrypsin (Alpha-1 protease inhibitor) (Alpha-1-antiproteinase) (Serpin A1) [Cleaved into: Short peptide from AAT (SPAAT)]
Gene: SERPINA1

Interactor 2: ELNE (P08246)
Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase)
Gene: ELANE

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.60	1.00	0.778

Property	Details
Experiments	10867014, 16321984
Identification Methods	pull down, comigration in non denaturing gel electrophoresis
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	Serpin~Trypsin
Common GO terms	GO:0002020 - protease binding GO:0005576 - extracellular region GO:0005615 - extracellular space GO:0062023 - collagen-containing extracellular matrix GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 418 Hs			1 267
+	Hs
		344148 A1AT_HUMAN (1-418): 0: 521: 100.00		KLK1_HUMAN (3-250): 1.71e-92: 275: 25.83
+	Hs
		344162 A1AT_HUMAN (1-418): 0: 521: 100.00		PRTN3_HUMAN (30-255): 9.34e-92: 273: 53.71
+	Mm
		430527 A1AT1_MOUSE (1-418): 7.71e-163: 465: 63.48		Q9Z1R9_MOUSE (8-249): 7.43e-106: 308: 32.41
+	Hs
		344265 AACT_HUMAN (20-417): 3e-164: 469: 43.56		ELNE_HUMAN (1-267): 1.67e-102: 300: 100.00
+	Hs
		344349 AACT_HUMAN (20-417): 3e-164: 469: 43.56		KLK13_HUMAN (32-261): 2.87e-97: 287: 27.76
+	Hs
		371463 IPSP_HUMAN (42-415): 4.23e-154: 443: 45.24		KLKB1_HUMAN (13-256): 4.19e-97: 299: 26.24
+	Mm
		430524 A1AT1_MOUSE (1-418): 7.71e-163: 465: 63.48		ELNE_MOUSE (1-259): 1.5e-92: 275: 73.08
+	Hs
		371455 IPSP_HUMAN (42-415): 4.23e-154: 443: 45.24		FA11_HUMAN (22-248): 2.11e-92: 286: 27.24
+	Hs
		439065 ILEU_HUMAN (49-415): 2.65e-145: 420: 29.92		ELNE_HUMAN (1-267): 1.67e-102: 300: 100.00
+	Mm
		1141519 SPB6_MOUSE (48-415): 1.81e-147: 425: 30.42		KLK8_MOUSE (3-250): 1.37e-92: 275: 26.59
+	Hs
		344178 A1AT_HUMAN (1-418): 0: 521: 100.00		GRAM_HUMAN (13-249): 3.07e-34: 125: 33.46
+	Hs
		344149 A1AT_HUMAN (1-418): 0: 521: 100.00		KLK3_HUMAN (26-246): 1.15e-27: 108: 32.51
+	Hs
		344256 A1AT_HUMAN (1-418): 0: 521: 100.00		KLK4_HUMAN (28-249): 1.94e-25: 102: 30.90
+	Hs
		344283 AACT_HUMAN (20-417): 3e-164: 469: 43.56		GRAM_HUMAN (13-249): 3.07e-34: 125: 33.46
+	Hs
		344266 AACT_HUMAN (20-417): 3e-164: 469: 43.56		CATG_HUMAN (25-247): 1.04e-33: 124: 36.71
+	Mm
		430526 A1AT1_MOUSE (1-418): 7.71e-163: 465: 63.48		CELA1_MOUSE (28-246): 1.93e-34: 126: 36.78
+	Hs
		486155 SPB9_HUMAN (49-415): 1.97e-159: 455: 33.60		GRAB_HUMAN (25-248): 1.79e-34: 126: 34.87
+	Hs
		839515 SPB9_HUMAN (49-415): 1.97e-159: 455: 33.60		GRAM_HUMAN (13-249): 3.07e-34: 125: 33.46
+	Hs
		439323 SPB4_HUMAN (48-415): 1.16e-148: 428: 32.31		CATG_HUMAN (25-247): 1.04e-33: 124: 36.71
+	Hs
		397629 IPSP_HUMAN (42-415): 4.23e-154: 443: 45.24		KLK3_HUMAN (26-246): 1.15e-27: 108: 32.51