HitPredict

Interactor 1: RAS2 (P01120)
Ras-like protein 2 (EC 3.6.5.2)
Gene: RAS2

Interactor 2: CDC25 (P04821)
Cell division control protein 25
Gene: CDC25

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.975

Property	Details
Experiments	1569942, 1632785, 8441380, 8325863, 8524097, 8206969, 21073870, 10747953, 21457714
Identification Methods	two hybrid, pull down, enzymatic study, affinity chromatography technology, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	Ras~RasGEF, Ras~RasGEF_N
Common GO terms	GO:0005634 - nucleus GO:0005789 - endoplasmic reticulum membrane GO:0005886 - plasma membrane GO:0007265 - Ras protein signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 322 Sc			1 1589
+	Sc
		350755 RAS1_YEAST (1-321): 1.11e-90: 274: 57.72		CDC25_YEAST (1-1589): 0: 2338: 100.00
+	Hs
		919681 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RGL1_HUMAN (1057-1586): 5.06e-128: 419: 20.53
+	Hs
		897964 RAP1B_HUMAN (8-171): 2.27e-60: 192: 60.00		RGL1_HUMAN (1057-1586): 5.06e-128: 419: 20.53
+	Dm
		442959 RAP1_DROME (8-171): 1.01e-84: 254: 59.15		Q86BI2_DROME (1064-1550): 1.15e-95: 329: 17.57
+	Dm
		442968 RAP1_DROME (8-171): 1.01e-84: 254: 59.15		Q9GR11_DROME (1064-1550): 1.25e-95: 329: 17.57
+	Dm
		442969 RAP1_DROME (8-171): 1.01e-84: 254: 59.15		Q9U4U5_DROME (1064-1550): 5.34e-93: 326: 17.57
+	Hs
		919657 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RPGF5_HUMAN (1051-1542): 7.4e-109: 360: 17.27
+	Hs
		919625 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RPGF1_HUMAN (1053-1555): 1.92e-103: 359: 24.60
+	Hs
		905428 RRAS2_HUMAN (6-172): 5.33e-59: 189: 58.68		RGL3_HUMAN (1055-1571): 3.72e-107: 360: 21.02
+	Hs
		919656 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RPGF4_HUMAN (1027-1544): 2.3e-101: 351: 17.09
+	Hs
		171541 RAP1B_HUMAN (8-171): 2.27e-60: 192: 60.00		RGL2_HUMAN (1071-1570): 2.16e-102: 348: 18.72
+	Hs
		171543 RRAS2_HUMAN (6-172): 5.33e-59: 189: 58.68		RGL2_HUMAN (1071-1570): 2.16e-102: 348: 18.72
+	Dm
		442976 RAP1_DROME (8-171): 1.01e-84: 254: 59.15		Q9VMF3_DROME (1285-1496): 1.02e-19: 98.6: 29.91
+	Ce
		1089983 RAP1_CAEEL (8-188): 1.1e-84: 254: 54.70		Q19770_CAEEL (1286-1561): 1.57e-19: 97.1: 25.98
+	Hs
		919618 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		GNDS_HUMAN (1109-1310): 1.67e-26: 120: 18.75
+	Hs
		897883 RAP1B_HUMAN (8-171): 2.27e-60: 192: 60.00		GNDS_HUMAN (1109-1310): 1.67e-26: 120: 18.75
+	Hs
		905420 RRAS2_HUMAN (6-172): 5.33e-59: 189: 58.68		GNDS_HUMAN (1109-1310): 1.67e-26: 120: 18.75
+	Hs
		919645 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RGDSR_HUMAN (1306-1543): 4.04e-21: 100: 29.89
+	Hs
		919687 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RPGF2_HUMAN (1279-1499): 2.14e-19: 97.4: 29.60
+	Hs
		919653 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RPGF6_HUMAN (1298-1500): 6.2e-19: 95.9: 29.41