HitPredict

Interactor 1: HSP82 (P02829)
ATP-dependent molecular chaperone HSP82 (82 kDa heat shock protein) (Heat shock protein Hsp90 heat-inducible isoform)
Gene: HSP82

Interactor 2: GCN2 (P15442)
eIF-2-alpha kinase GCN2 (EC 2.7.11.1) (General control non-derepressible protein 2) (Serine/threonine-protein kinase GCN2)
Gene: GCN2

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.73	1.00	0.856

Property	Details
Experiments	10567567, 15879519, 19536198
Identification Methods	coimmunoprecipitation, affinity chromatography technology, two hybrid, tandem affinity purification, two hybrid array
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	HSP90~Pkinase
Common GO terms	GO:0005524 - ATP binding GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 709 Sc			1 1659
+	Hs
		270364 HS90B_HUMAN (5-709): 0: 1026: 62.13		KS6A5_HUMAN (17-588): 1.76e-94: 327: 13.40
+	Hs
		270363 HS90A_HUMAN (4-709): 0: 1015: 60.83		KS6A5_HUMAN (17-588): 1.76e-94: 327: 13.40
+	Hs
		438011 HS90B_HUMAN (5-709): 0: 1026: 62.13		KS6A3_HUMAN (204-675): 9.63e-90: 311: 9.66
+	Hs
		438190 HS90B_HUMAN (5-709): 0: 1026: 62.13		KS6A2_HUMAN (612-981): 8.36e-89: 308: 18.11
+	Hs
		431672 HS90A_HUMAN (4-709): 0: 1015: 60.83		KS6A3_HUMAN (204-675): 9.63e-90: 311: 9.66
+	Hs
		438601 HS90B_HUMAN (5-709): 0: 1026: 62.13		KS6A6_HUMAN (205-570): 4.68e-86: 300: 12.02
+	Hs
		438193 HS90B_HUMAN (5-709): 0: 1026: 62.13		KS6A1_HUMAN (259-574): 3.22e-85: 298: 13.20
+	Hs
		431873 HS90A_HUMAN (4-709): 0: 1015: 60.83		KS6A2_HUMAN (612-981): 8.36e-89: 308: 18.11
+	Hs
		432343 HS90A_HUMAN (4-709): 0: 1015: 60.83		KS6A6_HUMAN (205-570): 4.68e-86: 300: 12.02
+	Hs
		272844 HS90B_HUMAN (5-709): 0: 1026: 62.13		KS6A4_HUMAN (17-539): 2.2e-81: 287: 13.32
+	Hs
		431876 HS90A_HUMAN (4-709): 0: 1015: 60.83		KS6A1_HUMAN (259-574): 3.22e-85: 298: 13.20
+	Hs
		272843 HS90A_HUMAN (4-709): 0: 1015: 60.83		KS6A4_HUMAN (17-539): 2.2e-81: 287: 13.32
+	Sc
		365561 HSP82_YEAST (1-709): 0: 984: 100.00		SNF1_YEAST (250-682): 7.43e-79: 276: 12.31
+	Hs
		438382 HS90B_HUMAN (5-709): 0: 1026: 62.13		NEK9_HUMAN (782-1023): 4.2e-20: 99.8: 28.18
+	Hs
		432092 HS90A_HUMAN (4-709): 0: 1015: 60.83		NEK9_HUMAN (782-1023): 4.2e-20: 99.8: 28.18
+	Hs
		438477 HS90B_HUMAN (5-709): 0: 1026: 62.13		E2AK1_HUMAN (248-543): 6.72e-11: 69: 17.74
+	Hs
		437881 HS90B_HUMAN (5-709): 0: 1026: 62.13		E2AK2_HUMAN (594-698): 2.86e-09: 63.9: 35.24
+	Hs
		432311 HS90A_HUMAN (4-709): 0: 1015: 60.83		E2AK3_HUMAN (588-686): 2e-11: 71.2: 42.06
+	Hs
		432211 HS90A_HUMAN (4-709): 0: 1015: 60.83		E2AK1_HUMAN (248-543): 6.72e-11: 69: 17.74
+	Hs
		431521 HS90A_HUMAN (4-709): 0: 1015: 60.83		E2AK2_HUMAN (594-698): 2.86e-09: 63.9: 35.24