HitPredict

Interactor 1: ESR1 (P03372)
Estrogen receptor (ER) (ER-alpha) (Estradiol receptor) (Nuclear receptor subfamily 3 group A member 1)
Gene: ESR1

Interactor 2: TRI25 (Q14258)
E3 ubiquitin/ISG15 ligase TRIM25 (EC 6.3.2.n3) (Estrogen-responsive finger protein) (RING finger protein 147) (RING-type E3 ubiquitin transferase) (EC 2.3.2.27) (RING-type E3 ubiquitin transferase TRIM25) (Tripartite motif-containing protein 25) (Ubiquitin/ISG15-conjugating enzyme TRIM25) (Zinc finger protein 147)
Gene: TRIM25

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.75	0.60	0.671

Property	Details
Experiments	31527615, 17418098, unassigned1953
Identification Methods	affinity chromatography technology, pull down, enzymatic study, anti tag coimmunoprecipitation, tandem affinity purification
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0043627 - response to estrogen GO:0051091 - positive regulation of DNA-binding transcription factor activity
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 595 Hs			1 630
+	Hs
		370380 ESR1_HUMAN (1-595): 0: 599: 100.00		TRIM8_HUMAN (7-358): 1.75e-77: 258: 25.21
+	Hs
		1052721 ESR2_HUMAN (26-582): 8.89e-136: 408: 45.88		TRI25_HUMAN (1-630): 3.52e-149: 447: 100.00
+	Hs
		175702 ESR2_HUMAN (26-582): 8.89e-136: 408: 45.88		TRI18_HUMAN (4-629): 4.7e-124: 383: 22.17
+	Hs
		487968 RARA_HUMAN (81-545): 1.35e-102: 320: 22.37		TRI25_HUMAN (1-630): 3.52e-149: 447: 100.00
+	Hs
		368914 ESR1_HUMAN (1-595): 0: 599: 100.00		TRI27_HUMAN (2-358): 2.11e-31: 130: 14.40
+	Hs
		370187 ESR1_HUMAN (1-595): 0: 599: 100.00		TRI11_HUMAN (6-288): 2.34e-31: 129: 19.08
+	Hs
		659746 RXRB_HUMAN (144-549): 1.9e-111: 345: 27.64		TRIM1_HUMAN (3-629): 8.74e-115: 361: 20.58
+	Hs
		1269384 ESR2_HUMAN (26-582): 8.89e-136: 408: 45.88		TRI47_HUMAN (11-630): 3.83e-81: 270: 23.71
+	Hs
		818879 RXRG_HUMAN (49-549): 4.82e-129: 388: 23.51		TRI67_HUMAN (48-591): 3.42e-69: 241: 15.73
+	Hs
		377659 GCR_HUMAN (5-557): 4.67e-109: 346: 22.51		TRIM8_HUMAN (7-358): 1.75e-77: 258: 25.21
+	Hs
		487428 ANDR_HUMAN (172-455): 8.95e-37: 148: 30.16		TRI25_HUMAN (1-630): 3.52e-149: 447: 100.00
+	Hs
		377417 GCR_HUMAN (5-557): 4.67e-109: 346: 22.51		TRI67_HUMAN (48-591): 3.42e-69: 241: 15.73
+	Hs
		659723 RXRB_HUMAN (144-549): 1.9e-111: 345: 27.64		TRI67_HUMAN (48-591): 3.42e-69: 241: 15.73
+	Hs
		536957 ESR2_HUMAN (26-582): 8.89e-136: 408: 45.88		TRI27_HUMAN (2-358): 2.11e-31: 130: 14.40
+	Hs
		536736 RXRB_HUMAN (144-549): 1.9e-111: 345: 27.64		TRI27_HUMAN (2-358): 2.11e-31: 130: 14.40
+	Hs
		536747 PPARG_HUMAN (55-546): 2.12e-100: 316: 20.35		TRI27_HUMAN (2-358): 2.11e-31: 130: 14.40
+	Hs
		487517 ANDR_HUMAN (172-455): 8.95e-37: 148: 30.16		TRI68_HUMAN (2-449): 3.97e-31: 128: 13.94