HitPredict

Interactor 1: RAF1 (P04049)
RAF proto-oncogene serine/threonine-protein kinase (EC 2.7.11.1) (Proto-oncogene c-RAF) (cRaf) (Raf-1)
Gene: RAF1

Interactor 2: RAP1A (P62834)
Ras-related protein Rap-1A (EC 3.6.5.2) (C21KG) (G-22K) (GTP-binding protein smg p21A) (Ras-related protein Krev-1)
Gene: RAP1A

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.88	1.00	0.940

Property	Details
Experiments	7862125, 7791872, 9867809, 9564038, 10454553, 8756332
Identification Methods	two hybrid, x-ray crystallography, pull down, affinity chromatography technology
Interaction Type	direct interaction, association, physical association

Property	Details
Interacting Pfam domains	C1_1~Ras, Pkinase~Ras, RBD~Ras
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 648 Hs			1 184
+	Hs
		373983 RAF1_HUMAN (1-648): 0: 652: 100.00		RAP1B_HUMAN (1-184): 6.73e-77: 229: 95.11
+	Hs
		374007 RAF1_HUMAN (1-648): 0: 652: 100.00		RAB1A_HUMAN (4-183): 2.18e-73: 221: 31.49
+	Hs
		374017 RAF1_HUMAN (1-648): 0: 652: 100.00		RAC1_HUMAN (1-184): 1.19e-73: 221: 29.08
+	Hs
		374854 RAF1_HUMAN (1-648): 0: 652: 100.00		RAB1B_HUMAN (4-179): 2.82e-72: 218: 30.51
+	Hs
		374003 RAF1_HUMAN (1-648): 0: 652: 100.00		RB11A_HUMAN (4-178): 2.1e-71: 216: 28.41
+	Hs
		373577 RAF1_HUMAN (1-648): 0: 652: 100.00		RAP2A_HUMAN (1-181): 3.65e-62: 192: 62.43
+	Hs
		349785 RAF1_HUMAN (1-648): 0: 652: 100.00		RASK_HUMAN (1-184): 8.24e-56: 176: 54.45
+	Hs
		349052 RAF1_HUMAN (1-648): 0: 652: 100.00		RASH_HUMAN (1-180): 7.38e-55: 173: 55.00
+	Hs
		373597 RAF1_HUMAN (1-648): 0: 652: 100.00		RALA_HUMAN (4-168): 1.19e-54: 173: 52.12
+	Rn
		442943 RAF1_RAT (1-648): 0: 648: 98.30		RASK_RAT (1-184): 3.12e-55: 174: 54.45
+	Rn
		507685 RAF1_RAT (1-648): 0: 648: 98.30		RASH_RAT (1-180): 7.38e-55: 173: 55.00
+	Mm
		699624 RAF1_MOUSE (1-648): 0: 647: 97.69		RASK_MOUSE (1-184): 3.12e-55: 174: 54.45
+	Mm
		1142565 RAF1_MOUSE (1-648): 0: 647: 97.69		RASH_MOUSE (1-180): 7.38e-55: 173: 55.00
+	Hs
		542845 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		RAP1B_HUMAN (1-184): 6.73e-77: 229: 95.11
+	Mm
		655977 BRAF_MOUSE (38-647): 3.22e-169: 503: 55.52		RAP1B_MOUSE (1-184): 6.73e-77: 229: 95.11
+	Hs
		542851 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		RAB1A_HUMAN (4-183): 2.18e-73: 221: 31.49
+	Hs
		542852 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		RAP1A_HUMAN (1-184): 5.85e-73: 219: 100.00
+	Hs
		349847 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		RASK_HUMAN (1-184): 8.24e-56: 176: 54.45
+	Hs
		349101 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		RASH_HUMAN (1-180): 7.38e-55: 173: 55.00
+	Mm
		655955 BRAF_MOUSE (38-647): 3.22e-169: 503: 55.52		RASH_MOUSE (1-180): 7.38e-55: 173: 55.00