HitPredict

Interactor 1: RAF1 (P04049)
RAF proto-oncogene serine/threonine-protein kinase (EC 2.7.11.1) (Proto-oncogene c-RAF) (cRaf) (Raf-1)
Gene: RAF1

Interactor 2: ILK (Q13418)
Scaffold protein ILK (ILK-1) (ILK-2) (Inactive integrin-linked kinase) (p59ILK)
Gene: ILK

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.75	1.00	0.871

Property	Details
Experiments	25852190, 26496610, 23455922
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation, tandem affinity purification
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	C1_1~PK_Tyr_Ser-Thr, Ank_2~Pkinase, PK_Tyr_Ser-Thr~Pkinase, PK_Tyr_Ser-Thr~RBD
Common GO terms	GO:0004672 - protein kinase activity GO:0005524 - ATP binding GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol and 1 others more... GO:0005886 - plasma membrane
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 648 Hs			1 452
+	Hs
		400118 RAF1_HUMAN (1-648): 0: 637: 100.00		LCK_HUMAN (167-449): 1.45e-109: 334: 21.99
+	Hs
		400209 RAF1_HUMAN (1-648): 0: 637: 100.00		SRC_HUMAN (173-448): 1.45e-98: 307: 24.56
+	At
		89197 F4I065_ARATH (2-643): 7.52e-170: 509: 17.05		Y5457_ARATH (3-452): 8.62e-124: 374: 15.15
+	At
		88402 Y3463_ARATH (2-632): 1e-160: 486: 17.21		Y2289_ARATH (4-448): 1.94e-126: 389: 14.85
+	At
		88739 Y3463_ARATH (2-632): 1e-160: 486: 17.21		Y4245_ARATH (9-448): 1.86e-124: 384: 14.02
+	At
		87682 Y1756_ARATH (2-638): 1.88e-158: 479: 15.62		SIRK_ARATH (9-450): 1.04e-126: 389: 15.48
+	At
		89236 RLK6_ARATH (2-643): 1.15e-163: 493: 15.77		Y5457_ARATH (3-452): 8.62e-124: 374: 15.15
+	At
		88338 Y2289_ARATH (10-644): 4.67e-156: 473: 17.06		SIRK_ARATH (9-450): 1.04e-126: 389: 15.48
+	At
		89259 Y3463_ARATH (2-632): 1e-160: 486: 17.21		Y5457_ARATH (3-452): 8.62e-124: 374: 15.15
+	At
		1422690 Y2451_ARATH (1-644): 6.6e-159: 480: 16.04		IOS1_ARATH (9-448): 3.47e-122: 378: 15.05
+	At
		87707 Y1756_ARATH (2-638): 1.88e-158: 479: 15.62		RLK0_ARATH (4-448): 1.01e-122: 379: 16.04
+	At
		88572 MEE39_ARATH (1-647): 1.27e-159: 482: 16.21		Y5457_ARATH (3-452): 8.62e-124: 374: 15.15
+	At
		89264 Y2451_ARATH (1-644): 6.6e-159: 480: 16.04		Y5457_ARATH (3-452): 8.62e-124: 374: 15.15
+	At
		87938 Y5189_ARATH (10-647): 3.25e-158: 479: 16.99		Y5457_ARATH (3-452): 8.62e-124: 374: 15.15
+	At
		1422672 IOS1_ARATH (2-643): 1.31e-154: 470: 15.97		IOS1_ARATH (9-448): 3.47e-122: 378: 15.05
+	At
		89221 BAK1_ARATH (77-647): 8.22e-158: 469: 17.30		Y5457_ARATH (3-452): 8.62e-124: 374: 15.15
+	Hs
		401599 RAF1_HUMAN (1-648): 0: 637: 100.00		M3K20_HUMAN (188-451): 5.69e-26: 113: 27.99