Interactor 1: SYFB (P07395)
Phenylalanine--tRNA ligase beta subunit (EC 6.1.1.20) (Phenylalanyl-tRNA synthetase beta subunit) (PheRS)
Gene: pheT

Interactor 2: SYFA (P08312)
Phenylalanine--tRNA ligase alpha subunit (EC 6.1.1.20) (Phenylalanyl-tRNA synthetase alpha subunit) (PheRS)
Gene: pheS

Species: Escherichia coli K-12

Source: intact

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.92 1.00 0.963

The method score is based on:

Property Details
Experiments 16606699, 24561554, 29176613, 6360212, 15690043, unassigned104, 16858726
Identification Methods affinity chromatography technology, two hybrid, molecular sieving, tandem affinity purification, pull down, two hybrid array, comigration in non denaturing gel electrophoresis, blue native page
Interaction Type physical association, direct interaction, association

The annotation score is based on:

Property Details
Interacting Pfam domains B3_4~tRNA-synt_2d, B5~tRNA-synt_2d, FDX-ACB~tRNA-synt_2d, Phe_tRNA-synt_N~tRNA_synthFbeta, tRNA-synt_2d~tRNA_synthFbeta
Common GO terms GO:0000049 - tRNA binding
GO:0000287 - magnesium ion binding
GO:0004826 - phenylalanine-tRNA ligase activity
GO:0005524 - ATP binding
GO:0005829 - cytosol
and 2 others

more...

Homologous interactions 10

Homologous interactions

Color coding for hit scores

1 795
Ec

1 327
+ Nv
+ Tt
+ Mp
+ Hs
+ Dm
+ Ce
+ Sc
+ Mt
+ Ec
+ Af

Service provided by Combinatics, Japan

Last updated: 15 Jul 2024