HitPredict

Interactor 1: UBC (P0CG48)
Polyubiquitin-C [Cleaved into: Ubiquitin]
Gene: UBC

Interactor 2: ITCH (Q96J02)
E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
Gene: ITCH

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.91	1.00	0.954

Property	Details
Experiments	38803224, 25723849, 30017913, 22496338, 23287719, 23644597, 19343052
Identification Methods	pull down, enzymatic study, unspecified method
Interaction Type	direct interaction, association

Property	Details
Interacting Pfam domains	HECT~ubiquitin
Common GO terms	GO:0005654 - nucleoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0016567 - protein ubiquitination GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 685 Hs			1 903
+	Hs
		476350 UBC_HUMAN (1-685): 0: 846: 100.00		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		125113 UBC_HUMAN (1-685): 0: 846: 100.00		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		476229 UBC_HUMAN (1-685): 0: 846: 100.00		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Hs
		475470 UBC_HUMAN (1-685): 0: 846: 100.00		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		475984 UBC_HUMAN (1-685): 0: 846: 100.00		HUWE1_HUMAN (415-902): 1.81e-160: 523: 34.88
+	Dm
		89879 UBIQP_DROME (1-610): 0: 752: 99.84		D5SHR3_DROME (484-903): 2.62e-171: 505: 45.31
+	Dm
		1376481 Q9W418_DROME (229-685): 0: 557: 99.78		Q9W325_DROME (496-903): 1.26e-168: 499: 46.38
+	Hs
		476414 UBC_HUMAN (1-685): 0: 846: 100.00		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		476403 UBC_HUMAN (1-685): 0: 846: 100.00		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Hs
		924156 RS27A_HUMAN (609-684): 7.23e-41: 148: 100.00		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		125347 RS27A_HUMAN (609-684): 7.23e-41: 148: 100.00		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Sp
		143368 UBI4P_SCHPO (305-685): 1.76e-151: 445: 95.80		PTR1_SCHPO (486-901): 1.43e-157: 514: 38.92
+	Hs
		475963 UBC_HUMAN (1-685): 0: 846: 100.00		HECW1_HUMAN (26-479): 2.25e-13: 76.9: 10.76
+	Sc
		477424 UBI4P_YEAST (381-685): 5e-122: 370: 95.74		TOM1_YEAST (487-902): 5.57e-156: 510: 39.10
+	Ce
		477557 UBIQ1_CAEEL (1-229): 5.97e-87: 292: 98.25		Q9GUP2_CAEEL (488-901): 1.03e-160: 523: 38.97
+	At
		1091758 UBQ3_ARATH (457-685): 3.23e-85: 272: 95.63		UPL1_ARATH (391-901): 4.26e-157: 513: 34.72
+	At
		1091762 UBQ3_ARATH (457-685): 3.23e-85: 272: 95.63		UPL2_ARATH (489-901): 3.99e-156: 510: 39.81
+	Mm
		924239 RS27A_MOUSE (609-684): 9.1e-41: 147: 100.00		HUWE1_MOUSE (415-902): 1.58e-160: 523: 34.88
+	Mm
		476858 UBC_MOUSE (1-108): 9.43e-30: 127: 90.74		HUWE1_MOUSE (415-902): 1.58e-160: 523: 34.88
+	Sc
		477293 UBI4P_YEAST (381-685): 5e-122: 370: 95.74		RSP5_YEAST (16-440): 9.75e-40: 160: 19.07