HitPredict

Interactor 1: DNAK (P0A6Y8)
Chaperone protein DnaK (HSP70) (Heat shock 70 kDa protein) (Heat shock protein 70)
Gene: DNAK

Interactor 2: NIKD (P33593)
Nickel import ATP-binding protein NikD (EC 7.2.2.11)
Gene: NIKD

Species: Escherichia coli K-12

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.62	0.60	0.612

Property	Details
Experiments	16606699, 15690043
Identification Methods	affinity chromatography technology, pull down, tandem affinity purification
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005524 - ATP binding GO:0005886 - plasma membrane GO:0016020 - membrane GO:0016887 - ATP hydrolysis activity
Homologous interactions	5

Homologous interactions

Color coding for hit scores

	1 638 Ec			1 254
+	Ec
		491174 DNAK_ECOLI (1-638): 0: 871: 100.00		METN_ECOLI (4-251): 1.21e-77: 239: 27.91
+	Ec
		491119 DNAK_ECOLI (1-638): 0: 871: 100.00		GLNQ_ECOLI (4-242): 8.51e-77: 234: 23.36
+	Ec
		491203 DNAK_ECOLI (1-638): 0: 871: 100.00		DPPF_ECOLI (3-251): 4.14e-71: 222: 29.48
+	Ec
		491193 DNAK_ECOLI (1-638): 0: 871: 100.00		YEJF_ECOLI (18-243): 4.75e-68: 220: 34.47
+	Ec
		491191 DNAK_ECOLI (1-638): 0: 871: 100.00		NIKE_ECOLI (16-221): 2.3e-24: 99.8: 35.81