HitPredict

Interactor 1: NCF1 (P14598)
Neutrophil cytosol factor 1 (NCF-1) (47 kDa autosomal chronic granulomatous disease protein) (47 kDa neutrophil oxidase factor) (NCF-47K) (Neutrophil NADPH oxidase factor 1) (Nox organizer 2) (Nox-organizing protein 2) (SH3 and PX domain-containing protein 1A) (p47-phox)
Gene: NCF1

Interactor 2: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Species: Homo sapiens

Source: biogrid

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.38	1.00	0.618

Property	Details
Experiments	30833792
Identification Methods	affinity chromatography technology
Interaction Type	physical association

Property	Details
Interacting Pfam domains	NECFESHC~SH3_1, SH3_1~p47_phox_C, SH2~SH3_1, SH3_1~SH3_1
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0017124 - SH3 domain binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 390 Hs			1 217
+	Hs
		111375 NCF1_HUMAN (1-390): 1.2e-102: 310: 100.00		J3KT38_HUMAN (159-211): 2.48e-12: 63: 37.04
+	Hs
		557655 NCK1_HUMAN (130-350): 9.29e-34: 131: 17.16		GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00
+	Hs
		924570 SRC8_HUMAN (4-282): 7.5e-33: 131: 12.67		GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00
+	Hs
		433732 MYO1E_HUMAN (3-283): 4.56e-53: 191: 15.20		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47
+	Hs
		557594 NCK1_HUMAN (130-350): 9.29e-34: 131: 17.16		NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14
+	Hs
		337337 NCK1_HUMAN (130-350): 9.29e-34: 131: 17.16		ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46
+	Hs
		557630 NCK1_HUMAN (130-350): 9.29e-34: 131: 17.16		ABL2_HUMAN (4-139): 9.61e-22: 94.7: 34.04
+	Hs
		789066 SRC8_HUMAN (4-282): 7.5e-33: 131: 12.67		ABL2_HUMAN (4-139): 9.61e-22: 94.7: 34.04
+	Mm
		337637 NCK1_MOUSE (130-350): 2.3e-33: 129: 17.16		ABL1_MOUSE (4-139): 4.07e-22: 95.9: 35.46
+	Hs
		411986 NCK1_HUMAN (130-350): 9.29e-34: 131: 17.16		FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58
+	Mm
		763403 NCK1_MOUSE (130-350): 2.3e-33: 129: 17.16		FYN_MOUSE (161-215): 4.44e-14: 72.3: 31.58
+	Hs
		433350 SRC8_HUMAN (4-282): 7.5e-33: 131: 12.67		YES_HUMAN (59-215): 4.17e-13: 69.2: 14.91
+	Hs
		412328 SH3G2_HUMAN (6-211): 5.16e-32: 125: 10.68		FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58
+	Hs
		522981 SRC8_HUMAN (4-282): 7.5e-33: 131: 12.67		SRC_HUMAN (161-215): 3.76e-11: 63.8: 31.58
+	Mm
		639446 SRC8_MOUSE (4-282): 1.73e-32: 130: 12.84		LYN_MOUSE (129-215): 2.66e-11: 64.2: 22.47
+	Hs
		1078900 SH3G2_HUMAN (6-211): 5.16e-32: 125: 10.68		ITSN1_HUMAN (137-216): 1.36e-12: 68.1: 25.00
+	Hs
		298944 SRBS2_HUMAN (163-283): 1.9e-20: 95.7: 21.15		ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46
+	Hs
		298959 SRBS2_HUMAN (163-283): 1.9e-20: 95.7: 21.15		ABL2_HUMAN (4-139): 9.61e-22: 94.7: 34.04
+	Hs
		208989 NCK2_HUMAN (161-327): 3.03e-19: 90.2: 14.98		NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14
+	Hs
		209001 NCK2_HUMAN (161-327): 3.03e-19: 90.2: 14.98		ABL2_HUMAN (4-139): 9.61e-22: 94.7: 34.04