HitPredict

Interactor 1: BRAF (P15056)
Serine/threonine-protein kinase B-raf (EC 2.7.11.1) (Proto-oncogene B-Raf) (p94) (v-Raf murine sarcoma viral oncogene homolog B1)
Gene: BRAF

Interactor 2: MP2K1 (Q02750)
Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (MKK1) (EC 2.7.12.2) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK 1)
Gene: MAP2K1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.98 1.00 0.993

The method score is based on:

Property Details
Experiments 28514442, 19371126, 27034005, 27684187, 9733801, 31015455, 31980649, 33961781, 32707033, 36585710, 20212043, 18332145, 16810323, 17979178, 26496610, 35271311, 16888650, 23934108, 25437913, unassigned2173 and 3 others

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Identification Methods affinity chromatography technology, enzymatic study, pull down, anti bait coimmunoprecipitation, protein kinase assay, tandem affinity purification, anti tag coimmunoprecipitation, surface plasmon resonance, fluorescence technology, molecular sieving and 1 others

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Interaction Type physical association, direct interaction, phosphorylation reaction, association

The annotation score is based on:

Property Details
Interacting Pfam domains C1_1~Pkinase, PK_Tyr_Ser-Thr~Pkinase
Common GO terms GO:0000165 - MAPK cascade
GO:0004672 - protein kinase activity
GO:0004674 - protein serine/threonine kinase activity
GO:0004708 - MAP kinase kinase activity
GO:0005524 - ATP binding
and 14 others

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Homologous interactions 20

Homologous interactions (Top 20)

Color coding for hit scores

1 766
Hs

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Last updated: 15 Jul 2024