HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: EPHB1 (P54762)
Ephrin type-B receptor 1 (EC 2.7.10.1) (ELK) (EPH tyrosine kinase 2) (EPH-like kinase 6) (EK6) (hEK6) (Neuronally-expressed EPH-related tyrosine kinase) (NET) (Tyrosine-protein kinase receptor EPH-2)
Gene: EPHB1

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.56	1.00	0.751

Property	Details
Experiments	9430661
Identification Methods	two hybrid, affinity chromatography technology, anti bait coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	SH2~fn3, PK_Tyr_Ser-Thr~SH2, PK_Tyr_Ser-Thr~SH3_1
Common GO terms	GO:0005783 - endoplasmic reticulum GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0048013 - ephrin receptor signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 984
+	Hs
		557643 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		EPHA5_HUMAN (1-981): 0: 1143: 55.78
+	Hs
		209018 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		EPHA4_HUMAN (13-983): 0: 1150: 59.10
+	Hs
		208997 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		EPHA8_HUMAN (19-981): 0: 1061: 51.78
+	Hs
		665558 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		EPHB2_HUMAN (19-981): 0: 1198: 73.76
+	Hs
		208996 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		EPHA2_HUMAN (17-978): 0: 1032: 46.58
+	Hs
		789068 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		EPHA7_HUMAN (1-981): 0: 1151: 56.96
+	Hs
		789049 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		EPHA4_HUMAN (13-983): 0: 1150: 59.10
+	Hs
		171284 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		EPHB6_HUMAN (2-969): 0: 896: 46.49
+	Hs
		789048 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		EPHB4_HUMAN (3-975): 0: 1021: 56.22
+	Ce
		247479 Q95PW9_CAEEL (1-376): 2.62e-70: 227: 38.69		VAB1_CAEEL (5-960): 0: 694: 30.06
+	Hs
		208998 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		UFO_HUMAN (131-963): 1.83e-174: 532: 23.30
+	Hs
		435526 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		IGF1R_HUMAN (390-883): 2.15e-141: 458: 30.71
+	Hs
		674354 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		UFO_HUMAN (131-963): 1.83e-174: 532: 23.30
+	Hs
		789061 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		TYRO3_HUMAN (131-964): 6.38e-160: 495: 19.28
+	Mm
		763403 NCK1_MOUSE (1-377): 7.48e-99: 300: 98.94		FYN_MOUSE (613-871): 2.5e-64: 228: 43.46
+	Hs
		411986 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		FYN_HUMAN (613-879): 2.52e-64: 228: 42.54
+	Hs
		788974 TEC_HUMAN (27-377): 4.7e-38: 146: 17.63		FRK_HUMAN (564-871): 2.44e-64: 228: 38.92
+	Hs
		442789 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		HCK_HUMAN (579-879): 7.25e-65: 229: 40.07
+	Hs
		412070 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		FYN_HUMAN (613-879): 2.52e-64: 228: 42.54
+	Rn
		88904 D4A3M8_RAT (1-376): 3.92e-90: 277: 66.93		INSR_RAT (338-495): 1.19e-09: 64.6: 15.64