HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: GIT1 (Q9Y2X7)
ARF GTPase-activating protein GIT1 (ARF GAP GIT1) (Cool-associated and tyrosine-phosphorylated protein 1) (CAT-1) (CAT1) (G protein-coupled receptor kinase-interactor 1) (GRK-interacting protein 1) (p95-APP1)
Gene: GIT1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.77	1.00	0.881

Property	Details
Experiments	31980649, 33961781, 36963288, unassigned2173
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Ank_2~SH3_1
Common GO terms	GO:0005829 - cytosol GO:0048013 - ephrin receptor signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 761
+	Hs
		209194 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		GIT1_HUMAN (1-761): 1.3e-176: 527: 100.00
+	Hs
		557688 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		GIT2_HUMAN (1-758): 2.93e-147: 451: 64.39
+	Hs
		209052 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		GIT2_HUMAN (1-758): 2.93e-147: 451: 64.39
+	Hs
		557823 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		557696 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ACAP1_HUMAN (2-297): 2.23e-56: 208: 23.77
+	Hs
		209187 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		195783 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ASAP2_HUMAN (2-506): 1.34e-50: 193: 15.20
+	Hs
		557775 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ARAP1_HUMAN (8-205): 6.26e-49: 188: 29.35
+	Hs
		195770 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ASAP2_HUMAN (2-506): 1.34e-50: 193: 15.20
+	Hs
		209145 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ARAP1_HUMAN (8-205): 6.26e-49: 188: 29.35
+	Hs
		557660 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ANK2_HUMAN (162-494): 7.25e-30: 129: 9.52
+	Hs
		789012 TEC_HUMAN (27-377): 4.7e-38: 146: 17.63		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		655502 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		412404 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		655450 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		AGAP2_HUMAN (2-223): 3.67e-52: 198: 32.00
+	Mm
		1112566 SRBS2_MOUSE (46-288): 1.38e-24: 107: 18.85		AGAP2_MOUSE (2-223): 9.71e-49: 188: 29.28
+	Hs
		195787 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		ASAP2_HUMAN (2-506): 1.34e-50: 193: 15.20
+	Hs
		412284 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		ARAP2_HUMAN (7-223): 6.18e-51: 195: 27.15
+	Hs
		195776 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		ASAP2_HUMAN (2-506): 1.34e-50: 193: 15.20
+	Mm
		103593 P85A_MOUSE (241-376): 1.59e-22: 101: 19.57		ANK3_MOUSE (237-740): 1.36e-20: 99.9: 9.25