HitPredict

Interactor 1: TBP (P20226)
TATA-box-binding protein (TATA sequence-binding protein) (TATA-binding factor) (TATA-box factor) (Transcription initiation factor TFIID TBP subunit)
Gene: TBP

Interactor 2: TF3B (Q92994)
Transcription factor IIIB 90 kDa subunit (TFIIIB90) (hTFIIIB90) (B-related factor 1) (BRF-1) (hBRF) (TAF3B2) (TATA box-binding protein-associated factor, RNA polymerase III, subunit 2)
Gene: BRF1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.971

Property	Details
Experiments	18391023, 30413534, 11809839, 26496610, 10921893, 7624363, 35271311, 16713569
Identification Methods	affinity chromatography technology, pull down, two hybrid, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	BRF1~TBP, TBP~TFIIB
Common GO terms	GO:0000995 - RNA polymerase III general transcription initiation factor activity GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0006352 - DNA-templated transcription initiation GO:0006383 - transcription by RNA polymerase III
Homologous interactions	13

Homologous interactions

Color coding for hit scores

	1 339 Hs			1 677
+	Dm
		589972 TBP_DROME (11-339): 2.81e-105: 314: 54.37		Q9VEL2_DROME (2-677): 0: 600: 42.31
+	Dm
		1099962 TRF_DROME (125-338): 2.16e-95: 284: 53.27		Q9VEL2_DROME (2-677): 0: 600: 42.31
+	Sc
		526960 TBP_YEAST (120-337): 9.51e-102: 301: 69.73		TF3B_YEAST (5-547): 4.54e-156: 465: 31.30
+	Sp
		571843 TBP_SCHPO (110-337): 1.22e-104: 308: 67.10		TF3B_SCHPO (4-550): 6.95e-154: 456: 34.36
+	Hs
		589827 TBP_HUMAN (1-339): 7.86e-144: 411: 100.00		TF2B_HUMAN (1-290): 1.85e-113: 345: 20.07
+	Mm
		661496 TBP_MOUSE (1-339): 8.88e-142: 405: 91.15		TF2B_MOUSE (1-290): 2.87e-113: 345: 20.07
+	Hs
		589923 TBP_HUMAN (1-339): 7.86e-144: 411: 100.00		BRF2_HUMAN (1-239): 1.17e-62: 216: 19.84
+	Dm
		661528 TRF_DROME (125-338): 2.16e-95: 284: 53.27		TF2B_DROME (6-290): 6.92e-112: 341: 21.05
+	Dm
		661530 Q8T052_DROME (128-338): 2.89e-78: 253: 37.56		TF2B_DROME (6-290): 6.92e-112: 341: 21.05
+	Hs
		913420 TBPL1_HUMAN (155-338): 4.89e-77: 235: 40.22		TF2B_HUMAN (1-290): 1.85e-113: 345: 20.07
+	Sc
		526959 TBP_YEAST (120-337): 9.51e-102: 301: 69.73		TF2B_YEAST (4-278): 6.89e-84: 269: 18.45
+	At
		286708 TBP2_ARATH (151-337): 3.81e-99: 292: 80.75		PTF2_ARATH (4-488): 2.72e-59: 210: 14.37
+	Pf
		905369 TBP_PYRFU (160-245): 2.93e-24: 99.6: 41.86		TF2B_PYRFU (4-277): 4.12e-81: 261: 26.17