HitPredict

Interactor 1: CFTR (P26361)
Cystic fibrosis transmembrane conductance regulator (CFTR) (ATP-binding cassette sub-family C member 7) (Channel conductance-controlling ATPase) (EC 5.6.1.6) (cAMP-dependent chloride channel)
Gene: CFTR

Interactor 2: NHRF1 (P70441)
Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (NHERF-1) (Ezrin-radixin-moesin-binding phosphoprotein 50) (EBP50) (Regulatory cofactor of Na(+)/H(+) exchanger) (Sodium-hydrogen exchanger regulatory factor 1) (Solute carrier family 9 isoform A3 regulatory factor 1)
Gene: Nherf1

Species: Mus musculus

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.56	0.60	0.584

Property	Details
Experiments	12403779, 21976599
Identification Methods	affinity chromatography technology, anti bait coimmunoprecipitation
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005902 - microvillus GO:0016020 - membrane GO:0016324 - apical plasma membrane GO:0030165 - PDZ domain binding GO:0051649 - establishment of localization in cell
Homologous interactions	15

Homologous interactions

Color coding for hit scores

	1 1476 Mm			1 355
+	Hs
		153866 CFTR_HUMAN (1-1476): 0: 1075: 78.33		NHRF1_HUMAN (1-355): 1.54e-75: 238: 83.89
+	Hs
		529166 CFTR_HUMAN (1-1476): 0: 1075: 78.33		NHRF2_HUMAN (4-182): 6.81e-21: 93.9: 36.61
+	Hs
		529099 CFTR_HUMAN (1-1476): 0: 1075: 78.33		SCRIB_HUMAN (7-161): 1.21e-12: 71.2: 20.12
+	Hs
		529235 CFTR_HUMAN (1-1476): 0: 1075: 78.33		NHRF3_HUMAN (144-264): 7.66e-11: 65: 26.45
+	Hs
		529050 CFTR_HUMAN (1-1476): 0: 1075: 78.33		DLG1_HUMAN (122-234): 1e-09: 61.9: 21.67
+	Hs
		529355 CFTR_HUMAN (1-1476): 0: 1075: 78.33		NHRF4_HUMAN (89-250): 8.03e-10: 61.9: 17.37
+	Rn
		1144126 MRP2_RAT (762-1462): 6.83e-58: 223: 15.31		NHRF1_RAT (1-355): 2.81e-79: 248: 92.16
+	Hs
		153846 MRP4_HUMAN (58-647): 5.83e-61: 232: 15.73		NHRF1_HUMAN (1-355): 1.54e-75: 238: 83.89
+	Hs
		153939 MRP2_HUMAN (781-1462): 1.27e-57: 222: 15.01		NHRF1_HUMAN (1-355): 1.54e-75: 238: 83.89
+	Hs
		67899 MRP4_HUMAN (58-647): 5.83e-61: 232: 15.73		PDZ1P_HUMAN (2-272): 1.85e-44: 159: 29.71
+	Hs
		179358 MRP4_HUMAN (58-647): 5.83e-61: 232: 15.73		NHRF2_HUMAN (4-182): 6.81e-21: 93.9: 36.61
+	Hs
		179356 MRP4_HUMAN (58-647): 5.83e-61: 232: 15.73		SCRIB_HUMAN (7-161): 1.21e-12: 71.2: 20.12
+	Hs
		179363 MRP4_HUMAN (58-647): 5.83e-61: 232: 15.73		NHRF3_HUMAN (144-264): 7.66e-11: 65: 26.45
+	Hs
		1132912 MRP2_HUMAN (781-1462): 1.27e-57: 222: 15.01		NHRF3_HUMAN (144-264): 7.66e-11: 65: 26.45
+	Hs
		1200079 MRP2_HUMAN (781-1462): 1.27e-57: 222: 15.01		NHRF4_HUMAN (89-250): 8.03e-10: 61.9: 17.37