HitPredict

Interactor 1: VPP1 (P32563)
V-type proton ATPase subunit a, vacuolar isoform (V-ATPase a 1 subunit) (V-ATPase 95 kDa subunit) (Vacuolar pH protein 1) (Vacuolar proton pump a subunit) (Vacuolar proton translocating ATPase subunit a 1)
Gene: VPH1

Interactor 2: VATD (P32610)
V-type proton ATPase subunit D (V-ATPase subunit D) (Vacuolar proton pump subunit D)
Gene: VMA8

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.81	0.60	0.700

Property	Details
Experiments	11592965, 19081055, 18467557, 37968396
Identification Methods	coimmunoprecipitation, anti tag coimmunoprecipitation, affinity chromatography technology, protein complementation assay, dihydrofolate reductase reconstruction
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000329 - fungal-type vacuole membrane GO:0007035 - vacuolar acidification GO:0016471 - vacuolar proton-transporting V-type ATPase complex GO:0046961 - proton-transporting ATPase activity, rotational mechanism GO:1902600 - proton transmembrane transport
Homologous interactions	5

Homologous interactions

Color coding for hit scores

	1 840 Sc			1 256
+	Hs
		1274160 VPP1_HUMAN (8-824): 0: 1220: 40.21		VATD_HUMAN (1-256): 7.15e-94: 277: 50.78
+	Hs
		1335305 VPP4_HUMAN (7-824): 0: 1198: 36.82		VATD_HUMAN (1-256): 7.15e-94: 277: 50.78
+	Hs
		1377794 VPP2_HUMAN (7-822): 0: 1152: 36.59		VATD_HUMAN (1-256): 7.15e-94: 277: 50.78
+	Hs
		1035692 VPP3_HUMAN (7-826): 0: 1034: 35.75		VATD_HUMAN (1-256): 7.15e-94: 277: 50.78
+	Sc
		696782 VPP2_YEAST (4-824): 0: 987: 49.10		VATD_YEAST (1-256): 4.03e-105: 306: 100.00