HitPredict

Interactor 1: GPN1 (P47122)
GPN-loop GTPase 1 (EC 3.6.5.-) (Essential PCL1-interacting ATPase 1) (GPN-loop GTPase NPA3) (Nucleolar preribosomal-associated protein 3)
Gene: NPA3

Interactor 2: GPN3 (Q06543)
GPN-loop GTPase 3 (EC 3.6.5.-)
Gene: GPN3

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.87	1.00	0.936

Property	Details
Experiments	10688190, 32985767, 36190433, 37968396, 23324351
Identification Methods	two hybrid, affinity chromatography technology, pull down
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	ATP_bind_1~ATP_bind_1
Common GO terms	GO:0003924 - GTPase activity GO:0005525 - GTP binding GO:0006606 - protein import into nucleus GO:0007064 - mitotic sister chromatid cohesion
Homologous interactions	10

Homologous interactions

Color coding for hit scores

	1 385 Sc			1 272
+	Sc
		812026 GPN1_YEAST (1-385): 0: 555: 100.00		GPN2_YEAST (3-261): 2.45e-111: 326: 32.71
+	Sc
		811996 GPN1_YEAST (1-385): 0: 555: 100.00		GPN1_YEAST (1-272): 1.3e-94: 285: 25.27
+	Hs
		1336406 GPN1_HUMAN (6-360): 9.49e-144: 414: 48.63		GPN3_HUMAN (1-269): 7.76e-131: 373: 43.17
+	Hs
		1334507 GPN1_HUMAN (6-360): 9.49e-144: 414: 48.63		GPN2_HUMAN (4-269): 6.53e-130: 372: 35.08
+	At
		1122850 QQT2_ARATH (6-321): 5.21e-135: 392: 51.56		QQT1_ARATH (4-258): 1.92e-116: 337: 34.63
+	Ce
		804442 GPN1_CAEEL (5-305): 1.25e-129: 377: 52.49		Q9XW68_CAEEL (3-272): 1.06e-114: 332: 41.91
+	Ce
		136322 GPN1_CAEEL (5-305): 1.25e-129: 377: 52.49		O01426_CAEEL (4-268): 1.88e-105: 308: 36.70
+	Dm
		1359121 Q9V3R3_DROME (2-274): 6.98e-113: 336: 50.73		Q9VI70_DROME (3-261): 6.6e-114: 330: 40.74
+	Dm
		1359123 Q9V3R3_DROME (2-274): 6.98e-113: 336: 50.73		Q9VU67_DROME (2-261): 3.8e-109: 319: 32.96
+	Sc
		987754 GPN3_YEAST (1-257): 6.46e-97: 291: 26.64		GPN2_YEAST (3-261): 2.45e-111: 326: 32.71