HitPredict

Interactor 1: UB2D1 (P51668)
Ubiquitin-conjugating enzyme E2 D1 (EC 2.3.2.23) ((E3-independent) E2 ubiquitin-conjugating enzyme D1) (EC 2.3.2.24) (E2 ubiquitin-conjugating enzyme D1) (Stimulator of Fe transport) (SFT) (UBC4/5 homolog) (UbcH5) (Ubiquitin carrier protein D1) (Ubiquitin-conjugating enzyme E2(17)KB 1) (Ubiquitin-conjugating enzyme E2-17 kDa 1) (Ubiquitin-protein ligase D1)
Gene: UBE2D1

Interactor 2: RNF5 (Q99942)
E3 ubiquitin-protein ligase RNF5 (EC 2.3.2.27) (RING finger protein 5) (Ram1 homolog) (HsRma1)
Gene: RNF5

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.82	1.00	0.911

Property	Details
Experiments	25416956, 32296183, 19549727, unassigned1304
Identification Methods	two hybrid, validated two hybrid, two hybrid prey pooling approach, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-C3HC4_3
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0006511 - ubiquitin-dependent protein catabolic process GO:0061630 - ubiquitin protein ligase activity GO:0070936 - protein K48-linked ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 147 Hs			1 180
+	Hs
		1307272 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00
+	Hs
		1292368 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		RN185_HUMAN (17-179): 7.02e-52: 166: 60.98
+	Hs
		849409 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		RN185_HUMAN (17-179): 7.02e-52: 166: 60.98
+	At
		715141 UBC9_ARATH (1-147): 8.78e-82: 238: 77.55		Q9LN67_ARATH (6-168): 4.25e-35: 124: 26.94
+	Hs
		849406 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRI11_HUMAN (14-148): 2.69e-31: 119: 17.78
+	Hs
		849314 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRI26_HUMAN (14-123): 1.73e-28: 112: 21.85
+	Hs
		1318532 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRI31_HUMAN (19-126): 9.14e-27: 106: 20.91
+	Hs
		1336390 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRI39_HUMAN (24-148): 5.72e-26: 104: 21.43
+	Hs
		849437 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRI31_HUMAN (19-126): 9.14e-27: 106: 20.91
+	Hs
		849453 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRI39_HUMAN (24-148): 5.72e-26: 104: 21.43
+	Hs
		1354526 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRI10_HUMAN (14-156): 5.89e-24: 99: 17.45
+	Hs
		584907 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		RO52_HUMAN (23-148): 9.12e-25: 101: 18.11
+	Hs
		849366 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRI22_HUMAN (20-126): 2e-24: 100: 19.64
+	Hs
		849475 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRI10_HUMAN (14-156): 5.89e-24: 99: 17.45
+	Mm
		919925 UB2D2_MOUSE (1-147): 7.7e-82: 238: 89.12		TRI17_MOUSE (13-148): 1.18e-24: 100: 13.10
+	Hs
		262201 UB2D4_HUMAN (1-147): 1.29e-82: 241: 91.84		TRIM3_HUMAN (3-109): 3.92e-21: 91.3: 19.84
+	Hs
		262161 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRIM3_HUMAN (3-109): 3.92e-21: 91.3: 19.84
+	Hs
		849438 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		TRIM7_HUMAN (1-101): 4.17e-21: 91.3: 23.48
+	Hs
		849487 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		CBLC_HUMAN (6-93): 1.05e-19: 87.1: 28.28
+	Hs
		749252 UB2D1_HUMAN (1-147): 3.24e-82: 239: 100.00		BRCA1_HUMAN (25-76): 3.12e-19: 86.4: 34.62