HitPredict

Interactor 1: IMA5 (P52294)
Importin subunit alpha-5 (Karyopherin subunit alpha-1) (Nucleoprotein interactor 1) (NPI-1) (RAG cohort protein 2) (SRP1-beta) [Cleaved into: Importin subunit alpha-5, N-terminally processed]
Gene: KPNA1

Interactor 2: NUP50 (Q9UKX7)
Nuclear pore complex protein Nup50 (50 kDa nucleoporin) (Nuclear pore-associated protein 60 kDa-like) (Nucleoporin Nup50)
Gene: NUP50

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.977

Property	Details
Experiments	26186194, 28514442, 32296183, 33961781, 25416956, 26496610, 35271311, unassigned1304, 31515488
Identification Methods	affinity chromatography technology, two hybrid, validated two hybrid, anti tag coimmunoprecipitation, two hybrid prey pooling approach, two hybrid array
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Arm~NUP50, Arm_3~NUP50
Common GO terms	GO:0005643 - nuclear pore GO:0005654 - nucleoplasm GO:0006606 - protein import into nucleus
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 538 Hs			1 468
+	Hs
		238747 IMA7_HUMAN (1-538): 6.51e-160: 467: 80.48		NUP50_HUMAN (1-468): 1.01e-131: 390: 100.00
+	Hs
		134978 IMA3_HUMAN (1-538): 7e-156: 456: 45.84		NUP50_HUMAN (1-468): 1.01e-131: 390: 100.00
+	Hs
		168678 IMA6_HUMAN (1-538): 1.39e-152: 449: 79.59		NUP50_HUMAN (1-468): 1.01e-131: 390: 100.00
+	Hs
		131527 IMA4_HUMAN (3-538): 1.73e-150: 443: 45.91		NUP50_HUMAN (1-468): 1.01e-131: 390: 100.00
+	Hs
		853515 IMA1_HUMAN (3-538): 1.64e-149: 441: 44.36		NUP50_HUMAN (1-468): 1.01e-131: 390: 100.00
+	At
		1280910 IMPA1_ARATH (4-538): 9.01e-160: 467: 52.78		NU50A_ARATH (3-468): 5.16e-44: 161: 17.60
+	At
		285384 IMPA4_ARATH (4-538): 2.4e-157: 461: 52.32		NU50A_ARATH (3-468): 5.16e-44: 161: 17.60
+	At
		1320502 IMPA6_ARATH (4-538): 3.58e-156: 458: 50.74		NU50A_ARATH (3-468): 5.16e-44: 161: 17.60
+	At
		97810 IMPA2_ARATH (4-538): 8.35e-156: 457: 51.30		NU50A_ARATH (3-468): 5.16e-44: 161: 17.60
+	Hs
		134921 IMA3_HUMAN (1-538): 7e-156: 456: 45.84		RGPD4_HUMAN (180-468): 1.25e-39: 154: 16.84
+	At
		138100 IMPA3_ARATH (4-538): 5.39e-153: 449: 50.47		NU50A_ARATH (3-468): 5.16e-44: 161: 17.60
+	Hs
		63862 IMA3_HUMAN (1-538): 7e-156: 456: 45.84		RGPD3_HUMAN (196-468): 4.76e-39: 153: 14.69
+	Hs
		134843 IMA3_HUMAN (1-538): 7e-156: 456: 45.84		RANG_HUMAN (316-466): 8.74e-42: 149: 19.38
+	Hs
		834694 IMA5_HUMAN (1-538): 9.01e-156: 457: 100.00		RBP2_HUMAN (212-468): 1.6e-36: 146: 17.60
+	Hs
		134854 IMA3_HUMAN (1-538): 7e-156: 456: 45.84		RBP2_HUMAN (212-468): 1.6e-36: 146: 17.60
+	Hs
		152769 IMA1_HUMAN (3-538): 1.64e-149: 441: 44.36		RGPD8_HUMAN (196-468): 2.51e-39: 154: 14.34
+	Hs
		853440 IMA1_HUMAN (3-538): 1.64e-149: 441: 44.36		RGPD5_HUMAN (196-468): 4.34e-39: 153: 14.34
+	Hs
		131396 IMA4_HUMAN (3-538): 1.73e-150: 443: 45.91		RANG_HUMAN (316-466): 8.74e-42: 149: 19.38
+	Hs
		131404 IMA4_HUMAN (3-538): 1.73e-150: 443: 45.91		RBP2_HUMAN (212-468): 1.6e-36: 146: 17.60
+	Hs
		834693 IMA1_HUMAN (3-538): 1.64e-149: 441: 44.36		RBP2_HUMAN (212-468): 1.6e-36: 146: 17.60