HitPredict

Interactor 1: TWF1 (P53250)
Twinfilin-1 (Twinfilin-A)
Gene: TWF1

Interactor 2: ACT (P60010)
Actin (EC 3.6.4.-)
Gene: ACT1

Species: Saccharomyces cerevisiae S288C

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.82	1.00	0.910

Property	Details
Experiments	9700161, 11604420, 16569665, 26458246
Identification Methods	cosedimentation in solution, comigration in non denaturing gel electrophoresis, affinity chromatography technology, pull down
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	Actin~Cofilin_ADF
Common GO terms	GO:0005884 - actin filament
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 332 Sc			1 375
+	Hs
		1122425 TWF2_HUMAN (1-330): 2.68e-117: 344: 23.30		ACTBL_HUMAN (2-375): 0: 687: 83.42
+	Hs
		891834 TWF2_HUMAN (1-330): 2.68e-117: 344: 23.30		ACTB_HUMAN (1-375): 0: 601: 88.80
+	Hs
		929897 TWF2_HUMAN (1-330): 2.68e-117: 344: 23.30		ACTG_HUMAN (1-375): 0: 600: 89.07
+	Hs
		891728 TWF1_HUMAN (1-330): 1.28e-114: 337: 23.01		ACTB_HUMAN (1-375): 0: 601: 88.80
+	Hs
		929835 TWF1_HUMAN (1-330): 1.28e-114: 337: 23.01		ACTG_HUMAN (1-375): 0: 600: 89.07
+	Hs
		932210 TWF2_HUMAN (1-330): 2.68e-117: 344: 23.30		ACTC_HUMAN (2-375): 0: 591: 87.17
+	Hs
		915411 TWF2_HUMAN (1-330): 2.68e-117: 344: 23.30		ACTA_HUMAN (2-375): 0: 589: 86.63
+	Hs
		932122 TWF1_HUMAN (1-330): 1.28e-114: 337: 23.01		ACTC_HUMAN (2-375): 0: 591: 87.17
+	Dm
		501533 TWF_DROME (1-332): 2.97e-112: 331: 23.73		ACT1_DROME (2-375): 0: 596: 89.31
+	At
		862533 ADF6_ARATH (5-126): 2.12e-42: 145: 16.67		ACT7_ARATH (3-375): 0: 646: 84.18
+	Mm
		578583 COF1_MOUSE (4-126): 1.36e-41: 144: 16.28		ACTB_MOUSE (1-375): 0: 601: 88.80
+	Rn
		799735 COF1_RAT (4-126): 3.1e-41: 143: 16.28		ACTB_RAT (1-375): 0: 601: 88.80
+	Hs
		618038 COF1_HUMAN (4-126): 4.86e-41: 142: 16.28		ACTB_HUMAN (1-375): 0: 601: 88.80
+	Hs
		618049 COF1_HUMAN (4-126): 4.86e-41: 142: 16.28		ACTG_HUMAN (1-375): 0: 600: 89.07
+	Sp
		501582 COFI_SCHPO (5-130): 5.98e-44: 149: 18.66		ACT_SCHPO (1-375): 0: 591: 89.60
+	Sc
		887656 COFI_YEAST (1-130): 6.43e-42: 144: 22.14		ACT_YEAST (1-375): 0: 594: 100.00
+	Hs
		618052 COF1_HUMAN (4-126): 4.86e-41: 142: 16.28		ACTC_HUMAN (2-375): 0: 591: 87.17
+	Hs
		618053 COF1_HUMAN (4-126): 4.86e-41: 142: 16.28		ACTS_HUMAN (2-375): 0: 590: 86.90
+	Hs
		618046 COF1_HUMAN (4-126): 4.86e-41: 142: 16.28		ACTA_HUMAN (2-375): 0: 589: 86.63
+	Hs
		618050 COF1_HUMAN (4-126): 4.86e-41: 142: 16.28		ACTH_HUMAN (2-375): 0: 587: 86.63