HitPredict

Interactor 1: UBE2N (P61088)
Ubiquitin-conjugating enzyme E2 N (EC 2.3.2.23) (Bendless-like ubiquitin-conjugating enzyme) (E2 ubiquitin-conjugating enzyme N) (Ubc13) (UbcH13) (Ubiquitin carrier protein N) (Ubiquitin-protein ligase N)
Gene: UBE2N

Interactor 2: TRIM1 (Q9UJV3)
Probable E3 ubiquitin-protein ligase MID2 (EC 2.3.2.27) (Midin-2) (Midline defect 2) (Midline-2) (RING finger protein 60) (RING-type E3 ubiquitin transferase MID2) (Tripartite motif-containing protein 1)
Gene: MID2

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.63	1.00	0.800

Property	Details
Experiments	19690564, 21143188
Identification Methods	two hybrid, affinity chromatography technology
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_UBOX
Common GO terms	GO:0005737 - cytoplasm GO:0016567 - protein ubiquitination GO:0043123 - positive regulation of canonical NF-kappaB signal transduction GO:0051092 - positive regulation of NF-kappaB transcription factor activity GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 152 Hs			1 735
+	Hs
		1356055 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		TRIM1_HUMAN (1-735): 0: 592: 100.00
+	Hs
		1151762 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		Q6GX22_HUMAN (21-735): 0: 550: 95.66
+	Hs
		175730 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		TRI18_HUMAN (21-717): 8.76e-175: 518: 72.60
+	Hs
		175653 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		TRI18_HUMAN (21-717): 8.76e-175: 518: 72.60
+	Hs
		1052807 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		TRI25_HUMAN (24-706): 9.41e-99: 319: 20.96
+	Hs
		849330 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		TRI25_HUMAN (24-706): 9.41e-99: 319: 20.96
+	Hs
		1333889 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		TRI45_HUMAN (29-379): 2.85e-68: 237: 20.59
+	Hs
		1318358 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		TRIM8_HUMAN (16-417): 5.14e-61: 216: 20.34
+	Hs
		1318738 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		TRIM2_HUMAN (8-524): 1.29e-57: 210: 17.77
+	Hs
		1317493 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		TRI54_HUMAN (184-400): 2.63e-53: 190: 23.96
+	Hs
		1313765 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		TRI62_HUMAN (114-458): 1.79e-32: 133: 15.47
+	Hs
		1336390 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		TRI39_HUMAN (21-389): 6.9e-27: 117: 12.81
+	Hs
		849366 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		TRI22_HUMAN (23-287): 2.15e-24: 109: 16.48
+	Hs
		537142 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		TRI27_HUMAN (16-340): 1.18e-23: 107: 14.15
+	Hs
		584907 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		RO52_HUMAN (16-316): 3.12e-24: 108: 13.86
+	Hs
		536760 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		TRI27_HUMAN (16-340): 1.18e-23: 107: 14.15
+	Hs
		135045 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		TRI38_HUMAN (16-269): 6.94e-21: 98.2: 17.12
+	Hs
		135012 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		TRI38_HUMAN (16-269): 6.94e-21: 98.2: 17.12
+	Hs
		734819 UB2D4_HUMAN (4-149): 5.39e-81: 236: 45.89		TRI23_HUMAN (28-272): 6.41e-17: 86.7: 24.71
+	Hs
		734563 UB2D1_HUMAN (4-149): 3.22e-80: 235: 45.21		TRI23_HUMAN (28-272): 6.41e-17: 86.7: 24.71