HitPredict

Interactor 1: 1433E (P62258)
14-3-3 protein epsilon (14-3-3E)
Gene: YWHAE

Interactor 2: MELK (Q14680)
Maternal embryonic leucine zipper kinase (hMELK) (EC 2.7.11.1) (Protein kinase Eg3) (pEg3 kinase) (Protein kinase PK38) (hPK38) (Tyrosine-protein kinase MELK) (EC 2.7.10.2)
Gene: MELK

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.65	0.60	0.628

Property	Details
Experiments	35271311, 36931259
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0016020 - membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 255 Hs			1 651
+	Xl
		223344 O57468_XENLA (1-255): 1.7e-152: 426: 96.47		Q804T2_XENLA (3-651): 0: 582: 27.90
+	Hs
		647862 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		MARK3_HUMAN (3-651): 0: 569: 27.97
+	Mm
		910231 1433E_MOUSE (1-255): 4.87e-154: 430: 100.00		MARK3_MOUSE (3-651): 0: 566: 27.40
+	Mm
		910234 1433E_MOUSE (1-255): 4.87e-154: 430: 100.00		MARK2_MOUSE (3-651): 0: 538: 25.75
+	Hs
		909730 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		MARK2_HUMAN (3-651): 0: 535: 25.07
+	Xl
		223345 O57468_XENLA (1-255): 1.7e-152: 426: 96.47		Q8QGV3_XENLA (3-651): 2.41e-180: 533: 25.03
+	Hs
		909916 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		MARK4_HUMAN (3-651): 1.44e-171: 509: 26.56
+	Dm
		90534 1433E_DROME (1-254): 2.95e-146: 411: 82.69		E1JGN0_DROME (2-651): 1.25e-173: 526: 27.13
+	Mm
		910308 1433E_MOUSE (1-255): 4.87e-154: 430: 100.00		MARK4_MOUSE (3-651): 3.91e-170: 505: 26.28
+	Mm
		910318 1433E_MOUSE (1-255): 4.87e-154: 430: 100.00		MARK1_MOUSE (3-651): 3.15e-169: 505: 24.00
+	Hs
		910106 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		MARK1_HUMAN (3-651): 8.9e-168: 501: 24.13
+	Hs
		883214 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		SIK1_HUMAN (9-547): 1.29e-140: 430: 26.61
+	At
		965360 14332_ARATH (4-255): 1.05e-147: 414: 74.41		CIPKC_ARATH (7-352): 2.9e-138: 414: 34.70
+	At
		788672 14333_ARATH (1-253): 3.45e-147: 413: 74.12		CIPKC_ARATH (7-352): 2.9e-138: 414: 34.70
+	At
		1280891 14337_ARATH (2-255): 7.73e-147: 412: 71.21		CIPKC_ARATH (7-352): 2.9e-138: 414: 34.70
+	Rn
		871214 1433E_RAT (1-255): 4.87e-154: 430: 100.00		AAPK1_RAT (10-290): 3.12e-76: 255: 46.85
+	Hs
		909507 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		AAPK1_HUMAN (10-290): 2.93e-76: 255: 46.85
+	Hs
		871354 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		AAPK2_HUMAN (10-324): 1.59e-74: 251: 43.61
+	Sc
		419854 BMH2_YEAST (4-242): 1.14e-134: 382: 77.08		SNF1_YEAST (11-266): 3.32e-71: 244: 49.42
+	Sc
		419824 BMH1_YEAST (2-243): 2.43e-134: 381: 75.72		SNF1_YEAST (11-266): 3.32e-71: 244: 49.42