HitPredict

Interactor 1: 1433E (P62258)
14-3-3 protein epsilon (14-3-3E)
Gene: YWHAE

Interactor 2: KSR1 (Q8IVT5)
Kinase suppressor of Ras 1 (EC 2.7.11.1)
Gene: KSR1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.73	1.00	0.855

Property	Details
Experiments	35271311, 36931259, 17979178
Identification Methods	affinity chromatography technology, proximity labelling technology, cross-linking study, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	14-3-3~PK_Tyr_Ser-Thr
Common GO terms	GO:0005737 - cytoplasm GO:0005783 - endoplasmic reticulum GO:0005829 - cytosol GO:0016020 - membrane
Homologous interactions	11

Homologous interactions

Color coding for hit scores

	1 255 Hs			1 923
+	Mm
		910254 1433E_MOUSE (1-255): 4.87e-154: 430: 100.00		KSR1_MOUSE (1-923): 0: 715: 84.42
+	Hs
		909702 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		KSR2_HUMAN (33-898): 0: 577: 45.45
+	At
		248524 14331_ARATH (4-245): 3.88e-144: 405: 75.41		SIRK_ARATH (96-881): 1.27e-164: 505: 12.92
+	Hs
		110869 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		H7C560_HUMAN (252-914): 9.21e-149: 460: 22.80
+	Hs
		542849 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		BRAF_HUMAN (252-914): 2.03e-148: 459: 22.80
+	Mm
		655938 1433E_MOUSE (1-255): 4.87e-154: 430: 100.00		BRAF_MOUSE (252-914): 2.44e-148: 458: 22.95
+	Hs
		488645 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		ARAF_HUMAN (261-899): 1.38e-148: 454: 24.55
+	Gg
		982842 1433E_CHICK (1-255): 4.87e-154: 430: 100.00		BRAF_CHICK (252-914): 3.87e-145: 450: 23.10
+	Hs
		373995 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		RAF1_HUMAN (229-908): 9.62e-143: 440: 22.03
+	Dm
		507708 1433E_DROME (1-254): 2.95e-146: 411: 82.69		KRAF1_DROME (251-908): 5.23e-117: 376: 20.48
+	Hs
		411661 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		LCK_HUMAN (591-890): 2.14e-99: 322: 24.59