HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: DOCK4 (Q8N1I0)
Dedicator of cytokinesis protein 4
Gene: DOCK4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.89	1.00	0.946

Property	Details
Experiments	28514442, 31980649, 33961781, 21706016, 21988832, unassigned2173
Identification Methods	affinity chromatography technology, two hybrid, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	SH2~SH3_2, SH3_1~SH3_2
Common GO terms	GO:0005730 - nucleolus GO:0005737 - cytoplasm GO:0005794 - Golgi apparatus GO:0005829 - cytosol GO:0005886 - plasma membrane and 1 others more... GO:0017124 - SH3 domain binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 1966
+	Hs
		1078864 ITSN1_HUMAN (137-216): 1.36e-12: 68.1: 25.00		DOCK4_HUMAN (1-1966): 0: 2081: 100.00
+	Hs
		924695 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		DOCK3_HUMAN (1-1963): 0: 1836: 53.65
+	Mm
		1141146 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		DOCK1_MOUSE (2-1795): 0: 1822: 36.38
+	Hs
		924565 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		DOCK1_HUMAN (2-1792): 0: 1798: 36.67
+	Hs
		924868 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		DOCK5_HUMAN (2-1790): 0: 1744: 34.82
+	Hs
		557736 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		DOCK3_HUMAN (1-1963): 0: 1836: 53.65
+	Hs
		337501 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		DOCK3_HUMAN (1-1963): 0: 1836: 53.65
+	Hs
		412257 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		DOCK3_HUMAN (1-1963): 0: 1836: 53.65
+	Hs
		548738 VAV_HUMAN (2-60): 8.95e-15: 74.6: 42.37		DOCK2_HUMAN (2-1771): 0: 1830: 35.49
+	Hs
		557689 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		DOCK1_HUMAN (2-1792): 0: 1798: 36.67
+	Hs
		412190 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		DOCK1_HUMAN (2-1792): 0: 1798: 36.67
+	Hs
		225367 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		MED14_HUMAN (1505-1958): 6.83e-62: 236: 18.20
+	Hs
		225343 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		MED14_HUMAN (1505-1958): 6.83e-62: 236: 18.20
+	Hs
		225326 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		MED14_HUMAN (1505-1958): 6.83e-62: 236: 18.20
+	Hs
		554292 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		MUC1_HUMAN (1657-1963): 2.99e-17: 90.6: 12.42
+	Hs
		225333 FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58		MED14_HUMAN (1505-1958): 6.83e-62: 236: 18.20
+	Hs
		411582 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		MUC1_HUMAN (1657-1963): 2.99e-17: 90.6: 12.42
+	Hs
		297808 LYN_HUMAN (129-215): 1.44e-11: 65: 22.47		K0754_HUMAN (1413-1966): 5.1e-27: 123: 10.58
+	Hs
		337335 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		MUC1_HUMAN (1657-1963): 2.99e-17: 90.6: 12.42
+	Hs
		782828 CSK_HUMAN (2-139): 3.92e-24: 100: 36.24		TENS1_HUMAN (1564-1964): 3.26e-11: 71.3: 17.78