HitPredict

Interactor 1: GNAI1 (P63096)
Guanine nucleotide-binding protein G(i) subunit alpha-1 (Adenylate cyclase-inhibiting G alpha protein)
Gene: GNAI1

Interactor 2: RIC8A (Q9NPQ8)
Synembryn-A (Protein Ric-8A)
Gene: RIC8A

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.86	1.00	0.927

Property	Details
Experiments	33961781, 12509430, 26186194, 28514442, 21988832
Identification Methods	affinity chromatography technology, two hybrid, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	G-alpha~Ric8
Common GO terms	GO:0005737 - cytoplasm GO:0005886 - plasma membrane GO:0007186 - G protein-coupled receptor signaling pathway GO:0007193 - adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
Homologous interactions	13

Homologous interactions

Color coding for hit scores

	1 354 Hs			1 531
+	Hs
		444795 GNAI3_HUMAN (1-354): 1.51e-179: 503: 93.79		RIC8A_HUMAN (1-531): 0: 763: 100.00
+	Hs
		391469 GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89		RIC8A_HUMAN (1-531): 0: 763: 100.00
+	Hs
		451041 GNAO_HUMAN (1-354): 4.45e-175: 491: 72.11		RIC8A_HUMAN (1-531): 0: 763: 100.00
+	Hs
		837648 GNAQ_HUMAN (1-353): 3.49e-167: 472: 52.26		RIC8A_HUMAN (1-531): 0: 763: 100.00
+	Hs
		670487 GNA11_HUMAN (1-353): 1.33e-162: 460: 51.13		RIC8A_HUMAN (1-531): 0: 763: 100.00
+	Hs
		580453 GNAZ_HUMAN (1-353): 2.73e-160: 454: 67.23		RIC8A_HUMAN (1-531): 0: 763: 100.00
+	Hs
		1053441 GNA13_HUMAN (2-353): 1.72e-155: 443: 39.17		RIC8A_HUMAN (1-531): 0: 763: 100.00
+	Hs
		391475 GNAI2_HUMAN (1-354): 3.51e-178: 499: 87.89		RIC8B_HUMAN (10-527): 0: 587: 42.06
+	Ce
		851068 GNAO_CAEEL (1-354): 2.29e-171: 482: 69.86		RIC8_CAEEL (13-530): 0: 601: 25.57
+	Ce
		1325710 GPA16_CAEEL (1-354): 3.03e-162: 459: 66.39		RIC8_CAEEL (13-530): 0: 601: 25.57
+	Hs
		837655 GNAQ_HUMAN (1-353): 3.49e-167: 472: 52.26		RIC8B_HUMAN (10-527): 0: 587: 42.06
+	Dm
		592748 GNAI_DROME (1-354): 1.9e-168: 475: 77.75		RIC8_DROME (10-513): 0: 564: 31.91
+	Hs
		926649 GNAS2_HUMAN (1-354): 3.5e-143: 412: 38.23		RIC8B_HUMAN (10-527): 0: 587: 42.06