HitPredict

Interactor 1: H33 (P84243)
Histone H3.3
Gene: H3-3A

Interactor 2: ASF1A (Q9Y294)
Histone chaperone ASF1A (Anti-silencing function protein 1 homolog A) (hAsf1) (hAsf1a) (CCG1-interacting factor A) (CIA) (hCIA)
Gene: ASF1A

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.972

Property	Details
Experiments	18971269, 27217568, 23828041, 33961781, 36089195, 14718166, 20504901, 23075851, 24209620
Identification Methods	affinity chromatography technology, proximity labelling technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	ASF1_hist_chap~Histone
Common GO terms	GO:0001649 - osteoblast differentiation GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0006334 - nucleosome assembly GO:0032991 - protein-containing complex and 1 others more... GO:0042692 - muscle cell differentiation
Homologous interactions	18

Homologous interactions

Color coding for hit scores

	1 136 Hs			1 204
+	Hs
		935389 H31_HUMAN (1-136): 3.08e-60: 183: 96.32		ASF1A_HUMAN (1-204): 2.31e-128: 361: 100.00
+	Mm
		935461 H31_MOUSE (1-136): 3.08e-60: 183: 96.32		ASF1A_MOUSE (1-204): 1.45e-128: 361: 99.51
+	Mm
		952765 H33_MOUSE (1-136): 1.12e-59: 182: 100.00		ASF1A_MOUSE (1-204): 1.45e-128: 361: 99.51
+	Gg
		952779 H33_CHICK (1-136): 1.12e-59: 182: 100.00		ASF1_CHICK (1-204): 2.31e-128: 361: 100.00
+	Hs
		1086358 H31T_HUMAN (1-136): 2.58e-59: 181: 93.38		ASF1A_HUMAN (1-204): 2.31e-128: 361: 100.00
+	Hs
		1174696 H32_HUMAN (1-136): 5.32e-59: 180: 97.06		ASF1A_HUMAN (1-204): 2.31e-128: 361: 100.00
+	Mm
		952267 H32_MOUSE (1-136): 5.32e-59: 180: 97.06		ASF1A_MOUSE (1-204): 1.45e-128: 361: 99.51
+	Gg
		952273 H32_CHICK (1-136): 5.32e-59: 180: 97.06		ASF1_CHICK (1-204): 2.31e-128: 361: 100.00
+	Hs
		935298 H31_HUMAN (1-136): 3.08e-60: 183: 96.32		ASF1B_HUMAN (1-204): 9.25e-121: 342: 70.59
+	Hs
		952660 H33_HUMAN (1-136): 1.12e-59: 182: 100.00		ASF1B_HUMAN (1-204): 9.25e-121: 342: 70.59
+	Hs
		1086330 H31T_HUMAN (1-136): 2.58e-59: 181: 93.38		ASF1B_HUMAN (1-204): 9.25e-121: 342: 70.59
+	Mm
		935463 H31_MOUSE (1-136): 3.08e-60: 183: 96.32		ASF1B_MOUSE (1-204): 7.93e-117: 332: 72.06
+	Mm
		952769 H33_MOUSE (1-136): 1.12e-59: 182: 100.00		ASF1B_MOUSE (1-204): 7.93e-117: 332: 72.06
+	Mm
		952269 H32_MOUSE (1-136): 5.32e-59: 180: 97.06		ASF1B_MOUSE (1-204): 7.93e-117: 332: 72.06
+	Hs
		828399 CENPA_HUMAN (9-133): 2.42e-45: 146: 50.76		ASF1B_HUMAN (1-204): 9.25e-121: 342: 70.59
+	Dm
		356097 H3_DROME (1-136): 5.32e-59: 180: 97.06		ASF1_DROME (1-203): 1.05e-97: 284: 56.88
+	Sp
		251626 H31_SCHPO (1-136): 1.12e-57: 177: 91.91		ASF1_SCHPO (1-169): 1.83e-84: 252: 51.43
+	Sc
		689932 H3_YEAST (1-136): 3.62e-54: 168: 90.44		ASF1_YEAST (1-168): 7.8e-88: 261: 54.76