HitPredict

Interactor 1: MP2K1 (Q02750)
Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (MKK1) (EC 2.7.12.2) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK 1)
Gene: MAP2K1

Interactor 2: SMAD2 (Q15796)
Mothers against decapentaplegic homolog 2 (MAD homolog 2) (Mothers against DPP homolog 2) (JV18-1) (Mad-related protein 2) (hMAD-2) (SMAD family member 2) (SMAD 2) (Smad2) (hSMAD2)
Gene: SMAD2

Species: Homo sapiens

Source: mint

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.48	0.60	0.541

Property	Details
Experiments	24412244
Identification Methods	two hybrid, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0008285 - negative regulation of cell population proliferation GO:0010628 - positive regulation of gene expression GO:0045893 - positive regulation of DNA-templated transcription
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 393 Hs			1 467
+	Rn
		250835 AKT1_RAT (39-389): 8.74e-121: 360: 19.16		SMAD2_RAT (1-467): 0: 674: 99.36
+	Hs
		625459 CDK2_HUMAN (65-367): 2.85e-123: 359: 24.27		SMAD2_HUMAN (1-467): 0: 675: 100.00
+	Hs
		681864 AKT1_HUMAN (39-389): 1.97e-120: 359: 19.44		SMAD2_HUMAN (1-467): 0: 675: 100.00
+	Mm
		682117 AKT1_MOUSE (39-389): 6.73e-121: 360: 19.16		SMAD3_MOUSE (1-467): 0: 617: 83.94
+	Rn
		812704 AKT1_RAT (39-389): 8.74e-121: 360: 19.16		SMAD3_RAT (1-467): 0: 617: 83.94
+	Hs
		625355 CDK2_HUMAN (65-367): 2.85e-123: 359: 24.27		SMAD3_HUMAN (1-467): 0: 617: 83.94
+	Hs
		681788 AKT1_HUMAN (39-389): 1.97e-120: 359: 19.44		SMAD3_HUMAN (1-467): 0: 617: 83.94
+	Hs
		805391 MP2K3_HUMAN (53-380): 1.39e-65: 214: 36.69		SMAD3_HUMAN (1-467): 0: 617: 83.94
+	Hs
		681832 AKT1_HUMAN (39-389): 1.97e-120: 359: 19.44		SMAD4_HUMAN (11-456): 4.53e-151: 443: 31.58
+	Rn
		250838 AKT1_RAT (39-389): 8.74e-121: 360: 19.16		SMAD4_RAT (11-456): 2.52e-150: 441: 31.58
+	Hs
		805424 MP2K3_HUMAN (53-380): 1.39e-65: 214: 36.69		SMAD1_HUMAN (1-467): 0: 565: 55.53
+	Hs
		1035612 STK26_HUMAN (65-367): 5.81e-103: 312: 31.80		SMAD4_HUMAN (11-456): 4.53e-151: 443: 31.58
+	Hs
		1035652 STK24_HUMAN (65-364): 1.58e-102: 312: 32.12		SMAD4_HUMAN (11-456): 4.53e-151: 443: 31.58
+	Rn
		291521 AKT1_RAT (39-389): 8.74e-121: 360: 19.16		SMAD7_RAT (13-443): 2.3e-111: 337: 25.00
+	Hs
		167333 AKT1_HUMAN (39-389): 1.97e-120: 359: 19.44		SMAD7_HUMAN (13-443): 2.55e-109: 331: 25.23
+	Hs
		167345 MP2K6_HUMAN (62-380): 7.07e-67: 216: 37.99		SMAD7_HUMAN (13-443): 2.55e-109: 331: 25.23
+	Hs
		167341 MP2K3_HUMAN (53-380): 1.39e-65: 214: 36.69		SMAD7_HUMAN (13-443): 2.55e-109: 331: 25.23