HitPredict

Interactor 1: RS27A (P62979)
Ubiquitin-ribosomal protein eS31 fusion protein (Ubiquitin carboxyl extension protein 80) [Cleaved into: Ubiquitin; Small ribosomal subunit protein eS31 (40S ribosomal protein S27a)]
Gene: RPS27A

Interactor 2: UBQL1 (Q9UMX0)
Ubiquilin-1 (Protein linking IAP with cytoskeleton 1) (PLIC-1) (hPLIC-1)
Gene: UBQLN1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.61	1.00	0.783

Property	Details
Experiments	32296183, unassigned1304
Identification Methods	two hybrid, two hybrid array, validated two hybrid, two hybrid prey pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UBA~ubiquitin, ubiquitin~ubiquitin
Common GO terms	GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005783 - endoplasmic reticulum GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	18

Homologous interactions

Color coding for hit scores

	1 156 Hs			1 589
+	Hs
		987259 RS27A_HUMAN (1-156): 3.57e-76: 225: 100.00		UBQL2_HUMAN (1-589): 9.56e-145: 433: 71.29
+	Hs
		987499 RL40_HUMAN (1-125): 5.35e-44: 142: 64.80		UBQL1_HUMAN (1-589): 1.24e-174: 509: 100.00
+	Dm
		583716 RS27A_DROME (1-156): 1.19e-72: 216: 91.03		Q9VWD9_DROME (30-582): 3.33e-135: 407: 47.39
+	Hs
		987494 RL40_HUMAN (1-125): 5.35e-44: 142: 64.80		UBQL2_HUMAN (1-589): 9.56e-145: 433: 71.29
+	Mm
		509314 UBC_MOUSE (1-119): 1.08e-36: 135: 67.48		UBQL2_MOUSE (1-589): 8.37e-141: 424: 65.47
+	Ce
		113201 RL40_CAEEL (1-125): 7.95e-42: 137: 64.00		UBQL_CAEEL (31-586): 1.67e-115: 354: 36.30
+	Dm
		583719 RS27A_DROME (1-156): 1.19e-72: 216: 91.03		Q9VXF9_DROME (37-317): 4.1e-51: 184: 17.93
+	Dm
		583697 RS27A_DROME (1-156): 1.19e-72: 216: 91.03		Q9V3W9_DROME (37-394): 1.43e-40: 154: 13.95
+	Hs
		986976 RS27A_HUMAN (1-156): 3.57e-76: 225: 100.00		RL40_HUMAN (37-126): 1.89e-34: 128: 30.77
+	Dm
		583653 RS27A_DROME (1-156): 1.19e-72: 216: 91.03		RL40_DROME (37-126): 7.86e-34: 126: 29.67
+	Mm
		509336 UBC_MOUSE (1-119): 1.08e-36: 135: 67.48		BAG6_MOUSE (24-536): 1.29e-49: 187: 16.32
+	Mm
		59252 UBC_MOUSE (1-119): 1.08e-36: 135: 67.48		DDI2_MOUSE (47-318): 5.97e-50: 179: 14.39
+	Sc
		436272 RS31_YEAST (1-151): 4.56e-61: 187: 82.78		RL40A_YEAST (37-129): 2.23e-31: 120: 28.72
+	Hs
		986975 RS27A_HUMAN (1-156): 3.57e-76: 225: 100.00		RS27A_HUMAN (37-123): 1.11e-12: 68: 30.68
+	Bt
		987512 RS27A_BOVIN (1-156): 3.57e-76: 225: 100.00		RS27A_BOVIN (37-123): 1.11e-12: 68: 30.68
+	Mm
		987305 RL40_MOUSE (1-125): 5.35e-44: 142: 64.80		RL40_MOUSE (37-126): 1.89e-34: 128: 30.77
+	Hs
		987318 RL40_HUMAN (1-125): 5.35e-44: 142: 64.80		RL40_HUMAN (37-126): 1.89e-34: 128: 30.77
+	Sc
		509884 RL40A_YEAST (1-125): 2.08e-42: 139: 64.00		RAD23_YEAST (45-419): 1.18e-31: 128: 17.73