HitPredict

Interactor 1: PAK2 (Q13177)
Serine/threonine-protein kinase PAK 2 (EC 2.7.11.1) (Gamma-PAK) (PAK65) (S6/H4 kinase) (p21-activated kinase 2) (PAK-2) (p58) [Cleaved into: PAK-2p27 (p27); PAK-2p34 (p34) (C-t-PAK2)]
Gene: PAK2

Interactor 2: RHOU (Q7L0Q8)
Rho-related GTP-binding protein RhoU (CDC42-like GTPase 1) (GTP-binding protein-like 1) (Rho GTPase-like protein ARHU) (Ryu GTPase) (Wnt-1 responsive Cdc42 homolog 1) (WRCH-1)
Gene: RHOU

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.62	1.00	0.789

Property	Details
Experiments	31871319, 32707033
Identification Methods	affinity chromatography technology, pull down
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	PBD~Ras, Pkinase~Ras
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0019901 - protein kinase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 524 Hs			1 258
+	Hs
		436282 PAK2_HUMAN (1-524): 0: 538: 100.00		RHOC_HUMAN (51-231): 3.2e-89: 264: 46.96
+	Hs
		1026939 PAK2_HUMAN (1-524): 0: 538: 100.00		RHOV_HUMAN (49-258): 3.82e-87: 260: 60.66
+	Hs
		892346 PAK2_HUMAN (1-524): 0: 538: 100.00		RAC3_HUMAN (48-223): 1.28e-83: 249: 57.96
+	Hs
		950964 PAK2_HUMAN (1-524): 0: 538: 100.00		RHOG_HUMAN (48-233): 5.28e-83: 248: 48.93
+	Hs
		925245 PAK2_HUMAN (1-524): 0: 538: 100.00		RAC1_HUMAN (50-234): 2.11e-79: 239: 56.76
+	Hs
		900433 PAK2_HUMAN (1-524): 0: 538: 100.00		RHOA_HUMAN (51-232): 1.24e-77: 234: 46.15
+	Hs
		545351 PAK2_HUMAN (1-524): 0: 538: 100.00		RAC2_HUMAN (50-233): 5.11e-77: 233: 56.52
+	Mm
		925809 PAK2_MOUSE (1-524): 0: 531: 97.14		RAC1_MOUSE (50-234): 2.11e-79: 239: 56.76
+	Hs
		893958 PAK2_HUMAN (1-524): 0: 538: 100.00		CDC42_HUMAN (50-236): 2.23e-74: 226: 58.82
+	Rn
		1145152 PAK2_RAT (1-524): 0: 530: 96.95		CDC42_RAT (50-236): 2.23e-74: 226: 58.82
+	Hs
		564507 PAK2_HUMAN (1-524): 0: 538: 100.00		RHOQ_HUMAN (50-221): 2.69e-66: 206: 56.40
+	Mm
		103567 G5E884_MOUSE (1-523): 2.64e-169: 491: 74.95		RAC2_MOUSE (48-234): 5.46e-83: 248: 55.08
+	Hs
		892345 PAK1_HUMAN (1-523): 6.81e-169: 490: 74.63		RAC3_HUMAN (48-223): 1.28e-83: 249: 57.96
+	Hs
		1026945 PAK2_HUMAN (1-524): 0: 538: 100.00		RHOJ_HUMAN (40-238): 2.64e-63: 199: 51.26
+	Mm
		103566 G5E884_MOUSE (1-523): 2.64e-169: 491: 74.95		RAC1_MOUSE (50-234): 2.11e-79: 239: 56.76
+	Hs
		545350 PAK1_HUMAN (1-523): 6.81e-169: 490: 74.63		RAC2_HUMAN (50-233): 5.11e-77: 233: 56.52
+	Mm
		103565 G5E884_MOUSE (1-523): 2.64e-169: 491: 74.95		CDC42_MOUSE (50-236): 2.23e-74: 226: 58.82
+	Rn
		723755 PAK1_RAT (1-523): 2.1e-169: 491: 75.14		CDC42_RAT (50-236): 2.23e-74: 226: 58.82
+	Mm
		103575 G5E884_MOUSE (1-523): 2.64e-169: 491: 74.95		RHOQ_MOUSE (50-221): 2.26e-66: 206: 56.98
+	Hs
		564505 PAK1_HUMAN (1-523): 6.81e-169: 490: 74.63		RHOQ_HUMAN (50-221): 2.69e-66: 206: 56.40